Sephadex G-75 Matrix: Cross-linked dextran Particle Size Wet: 90 µm-280 µm Partical Size Dry: 40 µm-120 µm pH Stability Working Range: 2-10 Flow Rate: < Darcy´s Law1) Pressure/Flow Specification: min 90 cm/h, bed height 10 cm, column 5 cm i.d....
Metallothioneins and other metalloproteins in cadmium-exposed rat liver, kidney, and spleen were separated both on SW 3000 and Sephadex G-75 columns. The former column was used as a gel permeation column equipped with a high-speed liquid chromatograph and the concentration of metals in eluate ...
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The molecular mass of IgE-BF was comprised between 10 to 20 kDa as estimated by gel filtration through a calibrated Sephadex G-75 column. After fractionation on 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer ... M Sarfati,Y Vanderbeeken,M Rubio-Trujillo,... - 《Eu...
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1) sephadex column chromatograph 凝胶柱层析1. SPA 1,SPA 2,SPA 3,have been obtained from spirulina platensis powder by alcohol-defatting,water-extracting,alcohol-precipitating and sephadex column chromatograph. 螺旋藻干粉经乙醇脱脂后用水提取,用乙醇沉淀,并经凝胶柱层析精制,获得了3 种平均相对分子质量...
1)Sephadex G-75 gelSephadex G-75凝胶 1.The crude enzyme power was further purified by Sephadex G-75 gel filtration chromatography and its specific activity was 1.43倍;粗酶经Sephadex G-75凝胶过滤层析后得到较纯的酶,酶的比活力为1。 2)Sephadex G chromatographySephadex G色谱 3)Sephadex G-200 Ge...
METHODS: Sephadex G-50 column filtration method and microcolumn centrifugation method were employed to separate the free drug and liposome. 方法:分别采用葡聚糖柱层析法、微柱离心法分离脂质体和游离药物,并进行方法学考察,优选出测定Nobiliside-A脂质体包封率的方法。2...
Dry DEAE Sephadex A-25 anion exchange material (0.10 g) was swelled overnight in 0.5 M aq. AcOH–NaOH pH 5 and prepared as a ‘mini-column’ of 7 mm diameter and 14 mm height. Due to the Sephadex material’s high affinity for water, no precautions against drying were needed during ...