The fit function chosen includes two sigmoid functions (or Monod curves): one to represent the gene expression and one to represent the continuous bacterial growth throughout the assay. The Monod equation models the growth of bacteria populations36. It is dependent on the growth rate of the bact...
Intravenous broad-spectrum antibiotics were started immediately after the sepsis screening and the first set of blood sample had been performed. Moreover, we fol- lowed the same classification of infection as in our previous study (5). Three categories of infective episodes were prospec- tively ...
In Escherichia coli, a common mechanism of cell death by bactericidal antibiotics involves the generation of highly reactive hydroxyl radicals via the Fenton reaction. Here we show that vitamin C, a compound known to drive the Fenton reaction, sterilizes cultures of drug- susceptible and drug-...
Azger Dusthackeer and Vanaja Kumar* Abstract Background: Phage lysin, extracted from three bacteriophages was used in place of antibiotics to control the overgrowth of normal flora in processed sputum samples leading to the sensitive detection of Mycobacterium tuberculosis using diagnostic luciferase repo...
Phage lysin, extracted from three bacteriophages was used in place of antibiotics to control the overgrowth of normal flora in processed sputum samples leading to the sensitive detection of Mycobacterium tuberculosis using diagnostic luciferase reporter phage assay (DLRPA). Methods A total of 129 sputum...
The appropriate volume of preculture to give the same amount of cells was then added to 100 ml of 50% LB media containing the same concentrations of appropriate antibiotics in a 250-ml flask. The cell growth was monitored for 72–96 h, and 0.5 mm IPTG was added at OD = 0.1 to 0.5 ...
P. aeruginosa also produces a group of multicolored redox-active antibiotics, phenazines, which have been identified as virulence factors (Mahajan-Miklos et al., 1999). Similarly, S. aureus possesses a number of thiol-based transcriptional regulators that sense ROS stress and coordinate responses ...
Phage lysin, extracted from three bacteriophages was used in place of antibiotics to control the overgrowth of normal flora in processed sputum samples leading to the sensitive detection of Mycobacterium tuberculosis using diagnostic luciferase reporter
pylori infection have been made under various conditions wherein sterilized animals and antibiotics are used, gastric acid is neutralized and the secretion of gastric juice is controlled, no satisfactory result has been obtained. Recently, it was reported that both nude and euthymic mice were ...
The agents can include a variety of different antibiotics, which can be provided to the microorganisms at a number of different concentrations in order to determine properties of the bacteria such as the minimum inhibitory concentration or the minimum bacteriocidal concentration. The microorganisms can ...