The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) uses the β-gal Substrate 4-Methylumbelliferyl β-D-galactopyranoside (4-MUG) to detect Senescence-Associated beta-galactosidase (SA-β-gal) activity. Upon binding to β-gal, 4-MUG is ...
Senescence beta-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) 价 格 电议 起批量 ≥1 Kit 最小起订电询 供货总量电询 发货地址上海市浦东新区 建议售价￥电议/Kit 更新日期 2024年12月25日 产品规格见优宁维官网 即时洽谈 立即询价 查看联系方式 收藏产品 发送留言 举报 VIP3年 上...
产品英文名称Cell Senescence Kit (β-Galactosidase Assay) 实验建议Abbkine细胞衰老检测试剂盒(β-半乳糖苷酶) 是一种基于衰老时,衰老相关β-半乳糖苷酶(senescence-associated β-galactosidase; SA-β-Gal) 在pH 6.0环境中活性上调,而对衰老细胞或组织进行检测的试剂盒 ...
Analysis of cellular senescence in culture in vivo: the senescence-associated beta- galactosidase assay. Curr Protoc Cell Biol 2005;Chapter 18:Unit 18.9.Bandyopadhyay D, Gatza C, Donehower LA, Medrano EE (2005) Analysis of cellular senescence in culture in vivo: the senescence-associated beta-...
Senescence β-Galactosidase Staining Kit(1)结合特异性抗原:抗体与其他免疫球蛋白分子区别,就在于抗体能与相应抗原发生特异性结合,在体内导致生理或病理效应;在体外产生各种直接或间接的可见的抗原抗体结合反应。抗体是靠其分子上的特殊的结合部位与抗原结合的。 (2)激活补体:抗体与相应抗原结合后,借助暴露的补体结合点...
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Cells were seeded 1 × 103cells/well in a µ-Slide 8 Well (Ibidi, 80826) chambered coverslip in both 2D and 3D. After culturing for four days, cells were washed twice with PBS. Thereafter, the β-galactosidase assay was performed using the Senescence beta-Galactosidase Staining Kit (...
Senescence-Associated-β-Galactosidase (SA-β-gal) Assay The SA-β-gal activity was measured using Senescence Assay Kit (ST429, Beyotime) based on the manufacturer’s instructions. Briefly, cells were incubated in ONPG at room temperature for 12 h then stained with the Staining Mixture at 37 ...
Beta-galactosidase assay The pMSCs were cultivated in a 12-well plate with a seeding density of 5.0 × 104per well for 48 h. To determine the senescence-associated beta-galactosidase (SA-β-gal) activity, a SA-β-gal staining kit (Cell Signaling Technology, Danvers, MA, USA) was us...
we have investigated the origin of senescence-associated-(beta)-galactosidase activity by modifying the intracellular and lysosomal pH of young and senescent human umbilical vein endothelial cells and examining the effect of these manipulations on the levels of activity, using a flow cytometric assay. ...