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Peptides were purified with a Jupiter Proteo90A Phenomenex column (2050 × 25.0 mm2) on an Armen Glider CPC preparatory phase HPLC over a 43 min gradient to yield 90–95% purity. The gradient solvent systems utilized Buffer A (H2O with 0.1% TFA) and Buffer B (acetonitrile (ACN...
In analytical Materials 2016, 9, 727 6 of 18 chromatography (conditions: column: Jupiter C18; flow rate: 1.0 mL/min; eluent A, 0.05% TFA in H2O MilliQ; eluent B, 0.05% TFA in CH3CN; gradient 0%–35% B in 35 min, detector at 214 nm) the conjugate SAP-RGD showed a tR = 21.7...