Potassium Channel Selectivity and Gating at the Selectivity Filter: Structural BasisSynonyms C-type inactivation ; Open-probability ; Relative conductance Definition The selectivity of an ion channel refers to its propensity to favor the permeation of a specific ion type. The process called gating ...
Figure 2.The KcsA potassium channel selectivity filter main chain structure (Zhouet al., 2001). The front and back subunits of the K+channel tetramer as well as the side chains of the residues shown have been removed for clarity. The side chain of amino acid residue threonine of the signat...
Liu, S., and Gonen, T. (2018). MicroED structure of the NaK ion channel reveals a Na+ partition process into the selectivity filter. Commun. Biol. 1:38. doi: 10.1038/s42003-018-0040-8Liu, S.; Gonen, T. MicroED structure of the NaK ion channel reveals a Na+ partition process ...
Numerous investigations of ion selectivity have been conducted over more than 50 years, yet the mechanisms whereby the channels select certain ions and reject others are not well understood21. The selectivity filter is a part of the protein forming a narrow tunnel inside the ion channel which is...
ion permeation pathway for Cl−, Br−, I−, and NO3−in the M427μ mutant of bCLC-k. The uncertainty error in (A) and (B) is calculated based on Monte Carlo bootstrapping and shown in shaded colors (almost unnoticeable).CThe selectivity filter of bCLC-k along with the ...
Ion channelSelectivity filterUltraviolet absorption spectroscopyElectronic transitionA correlation between the character of pharmacological activity and the energies of electronic transitions in some biologically active molecules, affecting the nervous system, has been found. In order to explain the correlation,...
Functional assays showed that it exhibited a high affinity for the Drosophila Shaker Kv channel and its mammalian counterpart—rat Kv1.2, but weak activity on rat Kv1.3 and almost no activity on rat Kv1.1. We also found that site 379 in the filter region is a key determinant in controlling...
In ion channels, "rings" of ionized side chains that decorate the walls of the permeation pathway often lower the energetic barrier to ion conduction. Using single-channel electrophysiological recordings, we studied the poorly understood ring of four glutamates (and one glutamine) that dominate this...
In many K + channels, prolonged activating stimuli lead to a time-dependent reduction in ion conduction, a phenomenon known as C-type inactivation. X-ray structures of the KcsA channel suggest that this inactivated state corresponds to a "constricted" conformation of the selectivity filter. However...
To examine the functional properties of these unique channel proteins, we reconstituted purified proteins into liposomes and carried out flux assays similar to those described previously15,21,22,23. Due to the lack of a consensus K+ selective filter sequence we used 22Na+ as the tracer ion in...