Column Selection for HPLC Method DevelopmentTony Taylor
whichmainlydependsontheaccumulationof experience. Generalrequirementsforsolventselectionofmobilephaseis: (1)thesolventshouldbeofhighpurity,solventandstationary phaseimmiscible,andcanmaintainthestabilityofthecolumn; (2)thedetectorperformanceanduseofsolventshouldmatch; (3)solventonthesolubilityofsamplesshouldbe...
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Chapter 5 Column Design SelectionThis chapter discusses the column design selection. In high-performance 1iquid chromatography (HPLC), the choice and construction of the column itself and in particular the column fittings might be of decisive importance for preparing successfully high-efficiency ...
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Elution 2: (Cholesterol esters) Hexane Another Bond Elut NH2 sorbent column is attached below the existing one to trap cholesterol that breaks through the first during triglyceride elution. Elution 3: (Triglycerides) Hexane containing 1% diethyl ether and 10% methylene chloride The Bond Elut NH2 ...
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HPLC and UHPLC Autosampler Selection Guide › HPLC and UHPLC Column Compartment Selection Guide › HPLC and UHPLC Detector Selection Guide › HPLC and UHPLC System Configurator › HPLC System Total Cost of Ownership Calculator ›
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