Objective A convenient strategy was developed to recycle selectable markers using Cre/loxP system for constructing Komagataella phaffii strains co-expressing multiple proteins. Results A plasmid in this strategy was generated from pPICZ alpha A with integration of lox71-Sh ble-lox66. Firstly, the ...
GuodongLiu,YinboQu, inBiotechnology Advances, 2019 5.2.1Maximizing the number of available selectable markers In addition to the commonly used dominant markers, various auxotrophic strains have been created inT. reesei(Derntl et al., 2015;Zhang et al., 2010). With the aid of Cre/loxPor β-...
Immunohistochemical expression of putative cancer stem cell markers aldehyde dehydrogenase 1, SOX2, CD44 and OCT4 in different grades of oral epithelial dy... Objectives: The hypothesized existence of cancer stem cells (CSC) and its markers aldehyde dehydrogenase 1 (ALDH1), CD44, SOX2 and OCT4 ...
Trends in BiotechnologyGressel, J., 1992a. Indiscriminate use of selectable markers-sowing wild oats. Trends Biotech. 10: 382.Gressel J: Indiscriminate use of selectable markers - sowing wild oats? Trends Biotechnol 1992, 10:382.Gressel, J. (1992) Indiscriminate use of selectable markers-sowing...
Excising selectable markers from transgenic plantsThis Month in Biotechnologydoi:10.1038/74379Natalie DeWitt NNature Biotechnology
Selection and use of appropriate promoters, selectable markers, and reporter genes has been helpful for development of efficient protocols for transgenic rice ... CCGV Laxmi - 《Biotechnology Advances》 被引量: 103发表: 2000年 CHAPTER 5–PLANT SELECTABLE GENES, REPORTER GENES AND PROMOTERS This con...
Next, to determine the general applicability of our split selectable marker systems, we demonstrate the utility of these systems in the model plants Arabidopsis and poplar by successfully stacking two reporters eYGFPuv and RUBY, using split Kanamycin or Hygromycin resistance markers. In conclusion, ...
A thiostrepton resistance gene and its mutants serve as selectable markers in Geobacillus kaustophilus HTA426 . Biosci Biotechnol Biochem , in press. doi:... K Wada,J Kobayashi,M Furukawa,... - 《Bioscience Biotechnology & Biochemistry》 被引量: 0发表: 2016年 A thiostrepton resistance gene and...
The salt-tolerance gene rstB under the control of the cauliflower mosaic virus 35S promoter was used as a selectable marker gene in the Agrobacterium tumef
In principle, using this techno- logy, any gene cluster could be inserted into any strain of E. coli, as long as a counter-selectable marker gene is included in the insertion. We are anticipating that our genomic integration method using counter-selectable markers will be widely applicable to...