Selectable marker and reporter genes. In: Potrykus I, Spangenberg G, eds. Gene transfer to plants. Heidelberg: Springer Verlag, 325-36.Schrott M.Selectable marker and reporter genes[A].In:Spangenbery P G (ed).Gene Transfer to Plants:A laboratory Manual[C].Berlin Springer,1995:325-336....
In subject area: Biochemistry, Genetics and Molecular Biology A negative selectable marker is a gene whose expression will sensitize cells to a specific drug or class of drugs. From: Encyclopedia of Cancer (Second Edition), 2002 About this pageSet alert ...
In leaves, roots and tubers (in the case of potato) of these plants, the marker gene protein product (NPTII) was present in low amounts compared to the levels found in control transgenic plants produced using a CaMV35S-gene as a selectable marker.Sebahattin zcan...
Selective markers are reporter genes included in the plasmid that is incorporated into the bacterial genome. This reporter gene can then be used to check if transfection was successful; for example an antibiotic marker gene followed by a growth medium which contains the anti...
The ability to stack multiple genes in plants is of great importance in the development of crops with desirable traits but can be challenging due to limited selectable marker options. Here we establish split selectable marker systems using protein splici
Following the production of transgenic plants, the selectable marker gene(s) used in the process are redundant, and their retention may be undesirable. They can be removed by exploiting segregation among the progeny of co-transformants carrying both the selectable marker gene and the effector transg...
pastoris, a plasmid vector was constructed using the toxic gene mazF as the counter-selectable marker under the control of PAOX1 of P. pastoris. A failure scenario happened that the DNA elements used for HR were always incorrectly assembled in the host strain E. coli. So, it was suspected ...
The E. coli phosphomannose isomerase (EcPMI) gene is widely used as a selectable marker gene (SMG) in mannose (Man) selection-based plant transformation. Although some plant species exhibit significant PMI activity and active PMIs were even identified in Man-sensitive plants, whether plant PMIs ...
A selectable marker, the ASA2 gene of Nicotiana tabacum, is provided for transforming plant cells. The ASA2 promoter sequence is also provided which is capable of directing tissue culture specific transcription of a downstream structural gene. Also disclosed are truncated forms of the ASA2 promoter...
The present invention relates to novel functional amdS genes from A. niger that can be used as dominant and bi-directional selection marker gene in the transformation of orga