Calcium flux was imaged using Jurkat cells loaded with Indo-1-AM for flow cytometry and Fluo-4-AM for TIRF microscopy. For TIRF imaging of early formed microclusters in Fig. 6, expression of fluorescently tagged protein in cells was gauged by epifluorescence before the cells made contact with...
The seawater was then transferred to acid-washed and autoclaved 125 mL serum bottles and sparged continuously with air (10 mL min−1). The volumetric flow through the millifluidic devices was maintained at a rate of 0.01 mL min−1using a syringe pump (Harvard Apparatus PHD Ul...
It shows that the three-phase flow boiling hear transfer coefficient increases with the augment of the heat flux,the flow rate of black liquor and the volume fractions of solid particles. 采用汽-液-固三相循环流化床新型蒸发器蒸发麦草浆黑液,讨论了热通量、流速和粒子体积分数对三相流沸腾传热系数的...