and a labeled streptavidin-biotin staining kit (DAKO LSAB kit, alkaline phosphatase system 40). They were incubated with the antibody at a dilution of 1:100 (in Tris-HCl buffer) for one hour at room temperature. The peroxidase reaction used 3,3-diaminobenzidine tetrahydrochloride as chromogen ...