Alkaline phosphatase vs. luciferase as secreted reporter molecules in vivo - Hiramatsu, Kasai, et al. - 2005 () Citation Context ...tion (Fig. 2A). To compare the linearity of Gluc reporter with respect to cell number and its sensitivity compared to SEAP, a well established assay for ...
QUANTI-Blue™ Solution can be used to detect and quantify AP or SEAP activity. Secreted embryonic alkaline phosphatase (SEAP), a truncated form of placental AP, is a widely used reporter gene. SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular ...
HEK-Blue™ Detection is a cell culture medium that allows the detection of SEAP (Secreted embryonic alkaline phosphatase) as the reporter protein is secreted by the cells. HEK-Blue™ Detection contains all the nutrients necessary for cell growth and a specific SEAP color substrate. The hydr...
分泌型碱性磷酸酶(SEAP)是人胎盘碱性磷酸酶的突变体,最大特点是能由表达细胞分泌到细胞外,检测时可在不破坏细胞的情况下,任意时间点取细胞培养上清液进行重复、动态的检测,且被检测细胞还可继续其他用途. 产品描述 开曼公司的分泌性碱性磷酸酶报告基因分析试剂盒(荧光法)提供了一个简单的化学发光法来检测转染细胞上清...
SEAP检测是最早、也是最快速的转染效率优化方法之一。 前几代NovaBright™分泌型胎盘碱性磷酸酶 (SEAP) 报告基因化学发光检测试剂盒广泛用于报告基因的检测,以测量稳定细胞系或转染的原代细胞的基因表达,包括基因敲除、RNA干扰的读取。该测试方法广泛用于病毒功能的检测,包括病毒基因表达检测、病毒复制、病毒融合性、病毒...
Introduction The NovaBright™ Secreted Placental Alkaline Phosphatase (SEAP) Enzyme Reporter Gene Chemiluminescent Detection System is a chemiluminescent reporter gene assay system designed for the rapid and sensitive detection of secreted placental alkaline phosphatase (SEAP) in cell culture media. SEAP is...
f Parental and HDLBP KO cells were transfected secreted alkaline phosphatase (SEAP) construct. SEAP signal was quantified in medium and normalized to the intracellular Firefly luciferase (Fluc) activity. Each experiment was carried out in five technical replicates. Data were presented as mean values...
Secreted alkaline phosphatase (SEAP) Basic informationMore.. Product Name:Secreted alkaline phosphatase (SEAP) Synonyms: CAS: MF: MW:0 EINECS: Mol File:Mol File Information Error Report Your Email: Browse by provinceSecreted alkaline phosphatase (SEAP)SuppliersGlobal suppliers ...
(B) Assays for NF-κB signaling in the reporter cells expressing secreted alkaline phosphatase (SEAP). HEK-hTLR2-pcDNA and HEK-hTLR2-CD44 were incubated for 7 hours with zymosan with or without rhTSG-6. Values are mean ± SD (n = 3; **P < .001). (C) Murine macrophages pre-...
Objective To establish a cell model of secreted alkaline phosphatase (SEAP) controlled by HCV internal ribosome entry site ( IRES ). 目的:构建HCVIRES调控外分泌性碱性磷酸酶(SEAP)基因表达的细胞模型。 2) secreted alkaline phosphatase(SEAP)