1.supplementary alignment supplementary alignment是指一条read的一部分和参考区域1比对成功,另一部分和参考区域2比对成功,参考区域1和参考区域2没有交集(或很少),那么一条read就会产生两条sam文件, 将其中的一条sam文件作为represent alignment,而另一条作为supplementary alignment (flag为2048)。 将上面的fastq文件去...
public boolean getNotPrimaryAlignmentFlag() { return isSecondaryAlignment(); } 代码示例来源:origin: com.github.samtools/htsjdk /** * Tests if this record is either a secondary and/or supplementary alignment; * equivalent to {@code (getNotPrimaryAlignmentFlag() || getSupplementaryAlignmentFlag()...
In support of RNA mutation and phylogenetic analysis, a web server (RNA-TVcurve) was designed based on this alignment-free RNA comparison algorithm. It provides three functional modules: 1) visualization of numerical representation of RNA secondary structure; 2) detection of single-point mutation ...
M. Structure-based alignment and consensus secondary structures for three HIV-related RNA genomes. PLoS Comput. Biol. 11, e1004230 (2015). Article PubMed PubMed Central Google Scholar Deigan, K. E., Li, T. W., Mathews, D. H. & Weeks, K. M. Accurate SHAPE-directed RNA structure ...
Supplementary Materials: Providing practice papers and revision notes tailored to the MOE syllabus. Innovative Teaching Methods Course Outline at eduKate Sengkang Tuition Center The Course Outline of eduKate Sengkang Tuition Center, where we are committed to providing top-quality education to help your ...
Experimental design and infection Results and discussion Parasitological results Conclusion Data availability Change history References Acknowledgements Funding Author information Ethics declarations Additional information Electronic supplementary material Rights and permissions About this article AdvertisementScientific...
Unlike IgG, MN50 GMTs for the beta variant were lower following the booster than those for the ancestral strain following the primary vaccination series (GMT 661 EU vs 1470 EU), in alignment with the known decreased neutralising responses for this variant. Although the worldwide prevalence of ...
In alignment Hilpert et al.’s (2023) approach to employing multiple measurement points, which we adapted to a task-level analysis, we also calculated network metrics for the first and second halves of the task. This approach was intended to reveal whether there were notable differences in the...
(n = 10).j, Alignment to the start and stop of pressing bouts reveals that peak timing is an artifact of averaging many trials. Bouts of pressing for an individual animal can be defined based on the trial-by-trial press rate to establish the start and stop (left, top, red hash ...
(Supplementary InformationSI22). P-values were calculated using a 2-sided students t-test assuming unequal variance for each gene. Genes with an RPKM below 0.3 were considered silent or potential noise of gDNA contamination. Genes that were silent (below 0.3 RPKM) in one strain but expressed ...