Search or jump to... Search code, repositories, users, issues, pull requests... Provide feedback We read every piece of feedback, and take your input very seriously. Include my email address so I can be contacted Cancel Submit feedback Saved searches Use saved searches to filter your...
GST fusion proteins were obtained according to the standard protocol. For in vitro binding assays, GST fusion proteins bound to the Glutathione Sepharose 4B were incubated with purified proteins. Then, the bound proteins were separated by electrophoresis and immunoblotted with indicated antibodies. RNA ...
S4C). Using this construct, we found cytosolic GST-pGls1 accumulation in Δsbh1Δsbh2 and sbh1S3A/T5A cells, confirming a translocation defect (Fig. S4D). This indicates that transport of Gls1 into the ER is dependent not only on the presence of Sbh1 but also on its phosphorylation ...
Each input is 2% equivalent volume of the lysate before GST-pull down. the plasma membrane. TNT formation is rapidly induced by apoptotic signaling40 and oxidative stress41 (within 30 min and 2 hours, respectively). The N-terminus of M-Sec might function as a switch for initiating ...
(c) under the Exchange Act (17 CFR 240.13e-4(c)) Securities registered pursuant to Section 12(b) of the Act: Title of each class Common Stock, $0.01 par value Trading Symbol(s) AMD Name of each exchange on which registered The Nasdaq Global Select Market Indicate by check mark ...
Strikingly, the Sec23a/Sec24a-R541A mutant bound to Sec22-GST no more tightly than to GST alone (compare, for example, lanes 15 and 16). These results correlate nicely with those from the packaging assay (Figures 1A and 1B) and establish that the inability of the C site mutant Sec24...
S.K. and M.Y.-K. prepared plasmid constructs and performed immunocytochemistry, cell fractionation assays, lipid cosedimentation assays, and GST-pull down assays; T.A. also prepared plasmid constructs; M.Y. carried out sample preparation, crystallization, structure determination, and protein-lipid ...
Positive-stranded RNA viruses modify host organelles to form replication organelles (ROs) for their own replication. The enteroviral 3A protein has been demonstrated to be highly associated with the COPI pathway, in which factors operate on the ER-to-Gol
"\n", "n_under_year = df.loc[df.n_days <= 365].shape[0]\n", "horizontal_limit = 365 / df.n_days.max()\n", "vertical_limit = n_under_year / 270\n", "\n", "g.set_xlim(0, df.n_days.max())\n", "g.set_ylim(0, 270)\n", "g.set_xticks([1, 10, 100, 1000...
The proteins were separated by SDSand IB using anti-GST antibodies. Right panel shows SOCS-box–like motif deletion reduced the capacity of LANA to induce polyubiquitylation. Saos-2 cells were transfected with WT LANA or its SOCS mutant. After 24-h transfection, cells were treated with MG132...