pCMV-SPORT6-SEC23B质粒是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC23B质粒TTTCCTGCGTTATCCCCTGATT...
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c Interaction between purified CIDEC/FSP27 and GST-Flag-tagged SNAREs using an in vitro GST pull-down assay followed by western blot. d Lipid mixing between adiposomes was measured from the development of FRET between NBD-labeled lipids and Rhod-labeled lipids. The NBD-adiposomes were ...
(H) The accumulation of DE-Cad (green) and Arm (red) in sec6 mutant cells relative to adjacent control cells indicated by asterisks. (I) Drosophila Sec15 interacts with Drosophila GST-Rab11 preferentially in its GTP bound form but not with GST-human Rab6 or GST. (J) Embryonic extracts...
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(e.g.,WD40, WRKY, LEAandGST), signal transduction-related genes (e.g., receptor kinase) and energy metabolism-related genes (e.g.,Plasma membrane ATPaseandBeta-glucosidase) (Table S1). These proteins have previously been reported to act either directly or indirectly in abiotic stress ...
While sbh1S3A/T5A cells accumulated significant amounts of GSTpGls1 in the cytosol (Fig. S4D), none of the proline-directed kinase mutants did (Fig. S4E). Our results suggest that despite their defect in Mns1ΔgpαF translocation, Δkns1,Δmck1, and cdc28-1 cells were competent ...
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Preincubation with Sys1p inhibited the ability of Sec23/24p to bind GST-Bet1p. Mutation of the critical diacidic motif (DxE) in the Sys1p peptide abrogated this inhibition, whereas mutation of a separate diacidic signal (ExE) had no effect. (B) Preincubation of Sec23/24p with peptides ...