RNA samples were amplified and labeled using the Low Input Fluorescent Linear Amplification kit (Agilent). Briefly, cDNA was first synthesized combining 300 ng of RNA with T7 promoter primers, 5× First Strand Buffer, 0.1 M DTT, 10 mM dNTP mix, MMLV RT and RNaseOUT. This reaction mix was...
SpAGS-overexpression induces endo-mesodermal lineages in sea star embryos.a–c1.5 μg/μl stock of SpAGS-GFP mRNA was injected into unfertilized eggs and the micromere-like cells were labeled with red fluorescent dextran (dye) during 8–32 cell stage (a). At Day 2, embryos were scored...
Full size image Morphological characteristics ofO. annularis(also called thelobed star coral) andO. faveolatacolonies (also called themountainous star coral), chosen for analysis in the present study at Playa Kalki on Curaçao, are consistent with previously published descriptions17,41,50and briefly ...
Directly above the BG96 is a clip for the main antenna, labeled A1. This connects to a wire that routes to a solder pad on the inside of the enclosure itself, which indicates that the main LTE antenna has been built into the body of the enclosure. To the right of the module is the...
Yes, ladies and gentlemen, the Arctic summer storm is still there. After approximately 48 hours (which is long for an Arctic summer storm) it has weakened some compared to yesterday or the day before, but as far as I can see sea level pressure in the centre of the storm is still aroun...
RNA samples were amplified and labeled using the Low Input Fluorescent Linear Amplification kit (Agilent). Briefly, cDNA was first synthesized combining 300 ng of RNA with T7 promoter primers, 5× First Strand Buffer, 0.1 M DTT, 10 mM dNTP mix, MMLV RT and RNaseOUT. This reaction mix was...
RNA samples were amplified and labeled using the Low Input Fluorescent Linear Amplification kit (Agilent). Briefly, cDNA was first synthesized combining 300 ng of RNA with T7 promoter primers, 5× First Strand Buffer, 0.1 M DTT, 10 mM dNTP mix, MMLV RT and RNaseOUT. This reaction mix was...
To thoroughly conduct a comprehensive assessment of the 3D U-Net model’s performance across different regions of the SCS, we selected four different areas, each with a range of 4° × 4°, labeled as boxes A, B, C, and D, as shown in Figure 7a. Box A (116.5° E–117.5° E, 19...
The panel labeled ‘Day 80’ shows the result of the transformation of the EC structure, which includes: (1) complete dissipation of the eddy A1, (2) progressing of all eddies to the northwest and merging of A2 with A3, and (3) formation of a dipole pair A2,3 + C1 abeam of Tuapse...