R21944-500 通用Buffer 5×考马斯亮蓝G-250(蛋白定量用) 500ml R21939-0.5 通用Buffer 5× Second Strand Buffer 0.5ml R21939-10 通用Buffer 5× Second Strand Buffer 10ml R21938-100 通用Buffer 10× Wash Buffer 100ml R21919-6×1.5 通用Buffer 10× PCR BufferⅡ﹠mgcl2 6×1.5ml R21919-2×75 ...
[自配] Wash Buffer TBS/T:1000ml1×TBS,0.1%Tween-20,100ml 10×TBS+ 1ml Tween-20+ 900ml ddH2O（参照Cell signal Technology Protoco）Blocking Buffer:（3%BSA的TBST或PBST）封闭1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk。[自配]Blocking buffer:150mlfor 150ml, add 15ml 10×TBS...
产品概述 Description Molecular biology grade, 10% SDS Solution is supplied in one bottle containing 500mL. The buffer is certified RNase-free, economical, and ready-to-use. SDS is used as a component of both hybridization and wash buffers for northern and Southern blots and has many...
[自配] Wash Buffer TBS/T:1000ml 1×TBS,0.1%Tween-20,100ml 10×TBS+ 1ml Tween-20+ 900ml ddH2O (参照Cell signal Technology Protoco) Blocking Buffer:(3%BSA的TBST或PBST)封闭 1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk。 [自配]Blocking buffer:150ml for 150ml, add 15ml 10...
[自配] Wash Buffer TBS/T:1000ml 1×TBS,0.1%Tween-20,100ml 10×TBS+ 1ml Tween-20+ 900ml ddH2O (参照Cell signal Technology Protoco) Blocking Buffer:(3%BSA的TBST或PBST)封闭 1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk。
5. 往线粒体沉淀中加入0.5 mL Wash Buffer重悬线粒体沉淀,4℃,1000 g 离心5 min。 6. 取上清,转移至新的离心管中,4℃, 12,000 g 离心10 min。弃上清,高纯度的线粒体沉淀在管底。 7. 用50 -100μL Store Buffer 或合适的反应缓冲液重悬线粒体沉淀,立即使用或-70℃保存。
[自配] Wash Buffer TBS/T:1000ml 1×TBS,0.1%Tween-20,100ml 10×TBS+ 1ml Tween-20+ 900ml ddH2O (参照Cell signal Technology Protoco) Blocking Buffer:(3%BSA的TBST或PBST)封闭 1X TBS, 0.1% Tween-20 with 5% w/v nonfat dry milk。
R21944-100 通用Buffer 5×考马斯亮蓝G-250(蛋白定量用) 100ml R21944-500 通用Buffer 5×考马斯亮蓝G-250(蛋白定量用) 500ml R21939-0.5 通用Buffer 5× Second Strand Buffer 0.5ml R21939-10 通用Buffer 5× Second Strand Buffer 10ml R21938-100 通用Buffer 10× Wash Buffer 100ml R21919-6×1.5...
R21944-500 通用Buffer 5×考马斯亮蓝G-250(蛋白定量用) 500ml R21939-0.5 通用Buffer 5× Second Strand Buffer 0.5ml R21939-10 通用Buffer 5× Second Strand Buffer 10ml R21938-100 通用Buffer 10× Wash Buffer 100ml R21919-6×1.5 通用Buffer 10× PCR BufferⅡ﹠mgcl2 6×1.5ml R21919-2×75 ...
9.浓洗涤液(Wash Buffer):1×20ml/瓶,使用时每瓶用蒸馏水稀释25倍。 10.终止液(Stop Solution):1×10ml/瓶(2N H2SO4)。 操作注意事项: (1)SDS检测试剂盒多少钱试剂应按标签说明书储存,使用前恢复到室温。稀稀过后的标准品应丢弃,不可保存。