Find SDS-PAGE recipes for stacking gel, separating gel and buffer recipes. Essential for western blotting.
6. Load 10ul - 25ul (10ul for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15ul sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber by carefully adding 1 x SDS ...
Transfer the Buffer Don’t you hate it when your running buffer leaks out of the inner chamber? If the level of the buffer falls enough, your wells will get dry and your gel won’t run correctly. A tip that saved my electrophoresis: if you are losing buffer, just stop the electrophores...
By combining any of the NuPAGE® Novex Bis-Tris Gels with the MES SDS or MOPS SDS Running Buffer, you can obtain six separation ranges for resolving proteins over a wide molecular weight range of 1-200 kDa. The NuPAG...
The samples must be prepared with a TEO-Cl based sample buffer which is available as a premade 4X solution, or can be homemade with the freely available recipe.RunBlue Protein Gels have an extended shelf life of 2 years at 4ºC which is ideal for large stocks or infrequent use. They...