SDS sample buffer 1x (clear) pH 6.8 250 ml total; Trizma base1.9 g; Glycerol 31.42g; SDS 5.75 g; DDW to 250 ml 2X SDS SB: As above and then to final volume of 125ml; pH with concentrated HCl. NOTE. Add the HCl slowly once the pH reaches ~7.2 as it will fall rapidly thereaft...
SDS-PAGE异常电泳现象及分析SDS-PAGE Hall of Shame 2006-4 raimi编辑整理於 DICP SDS-PAGE "Hall of Shame"You may very well have prepared a nearly perfect gel, and would have a difficult time improving upon the product. If that is so, then by all means gloat about it! If you didn't ...
In addition, we learned that untreated samples (samples that are not heated or treated with urea) may look different on Bis-Tris gel depending on gel composition (non-gradient vs. gradient) and the buffer used (MES vs. MOPS). In certain case, the apparent lack of artifact bands on ...