Dr. Redfield seemed well beyond "retirement-age" , so this may explain his ineptness in communicating with me and perhaps explain why he thought my blood labs were too difficult to decipher. At the closing juncture of my appt I felt that I got up early, drove 35 miles through rush-hou...
For timers, Env protein was extracted from the supernatants using a Galanthus nivalis lectin (GNL) column (Vector Labs), whereas for nanoparticles, Env-fusion protein was purified using a 2G12 affinity column. Trimers might be further purified by size exclusion chromatography (SEC) on a Superdex...