After electroporation of the plasmid library, total RNA was extracted for RNA-seq library generation targeting the tested 3′ UTRs (Fig.1a, Supplementary Fig.2a). A DNA-seq library is also needed to allow for no
Cells were then rinsed and incubated with ice-cold catalase-treated PBS (1 mL/well) containing 100 mM NEM for 10 min on ice. Catalase-treated PBS was prepared by adding 5 μL pre-warmed (37 °C for 30 min) bovine catalase solution to 2.89 mL 50 mM pre-warmed potassium ...
While the numbers of hepatitis-C-virus (HCV) infections among men who have sex with men (MSM) who are co-infected with the human immunodeficiency virus (HIV) are on the rise, with vast evidence for sexual transmission of HCV in this population, concerns have also been raised regarding sexua...
While hepatotoxicity-assessment assays are usually part of early safety testing using liver-derived cell lines such as HepG2 or HepaRG cells, the in vitro cellular systems commonly applied in these experiments are not suitable for assessing toxic bile accumulation and cholestatic cytotoxicity [17-18...
Hit compounds were further validated via dose–response assays, with lead candidates then assessed for nematocidal activity against H. contortus adult worms, and additionally, for cytotoxic and mitotoxic effects on human hepatoma (HepG2) cells. Results The primary screen against H. contortus and C...
Robier C, Amouzadeh-Ghadikolai O, Stettin M, Reicht G. Comparison of the clinical utility of the Elia CTD Screen to indirect immunofluorescence on Hep-2 cells. Clin Chem Lab Med (2016) 54(8):1365-70. doi:10.1515/ cclm-2015-1051...
prognostic relevance for human breast cancer K Heilmann, R Toth, C Bossmann, K Klimo, C Plass and C Gerhauser The majority of long noncoding RNAs (lncRNAs) is still poorly characterized with respect to function, interactions with protein- coding genes, and mechanisms that regulate their ...
16. Cen C, Wang W, Yu S, Tang X, Liu J, Liu Y, Zhou L, Yu J, Zheng S. Develop- ment and validation of a clinical and laboratory-based nomogram to predict nonalcoholic fatty liver disease. Hep Intl. 2020;14:808–16. 17. Fedchuk L, Nascimbeni F, Pais R, Charlotte F, Hous...
Delgado, C. Vargas, L. Gil Tablets for all? Testing the screen inferiority effect with upper primary school students Learning and Individual Differences, 86 (2021), 10.1016/j.lindif.2021.101975 Google Scholar Schmuck et al., 2023 D. Schmuck, A. Stevic, J. Matthes, K. Karsay Out of ...
b Normalisr recovered uniformly distributed null P values for DE, as measured by the violin plot of P values of KS test (X) on null P values separately for each gene bin (in a). Bars show extrema. c Normalisr was much faster than most other methods. d Normalisr had uniformly ...