(B) Silver-stained SDS-PAGE gel-containing proteins derived from RNA pulldown by circSCP2 probe and negative control. (C) Western blotting analysis of protein precipitates obtained from RNA pulldown by circSCP2 probe and negative control. (D) qRT-PCR analysis of circSCP2 expression in anti-...
Cell extracts containing 50 μg of protein were subjected to SDS-PAGE (Boster, Wuhan, China, AR0138) and transferred to PVDF membranes (Millipore, ISEQ00010). After blocking, membranes were incubated overnight with primary antibodies against SCP2 (1:1000), PKA (1:2500), SUFU (1:1000)...
(B) Silver-stained SDS-PAGE gel-containing proteins derived from RNA pulldown by circSCP2 probe and negative control. (C) Western blotting analysis of protein precipitates obtained from RNA pulldown by circSCP2 probe and negative control. (D) qRT-PCR analysis of circSCP2 expression in anti-...
The sterol carrier protein-2 (SCP-2) family includes proteins containing a sterol carrier domain (SCP-2 domain). The founding member of this family, SCP-2 itself, is a low molecular mass (12–15 kDa) single-domain protein. SCP-2 was first identified as an intracellular nonspecific lipid ...
it cannot be excluded that certain functions of SCP2 are taken over by other proteins containing this domain (e.g. MFP2) or that otherlipidbinding proteins, as shown for FABP[38], are upregulated. Spontaneousperoxisomeproliferation is seen in liver ofScpx/Scp2knockout mice.VLCFAare normal, ...
The SCPx mRNA spans two exons in genome, and conceptual translation of the SCPx cDNA encodes a protein of 536 amino acids, which contains a thiolase domain and a SCP2 domain. Whereas the SCP2 mRNA partly lakes the first exon, and the SCP2 is a 146 amino acids containing a SCP2 domain...
(59/40/1 IPA/HEX, H2O containing 10 mM NH4oAC). For LC–MS analysis, the samples were reconstituted in 50 µl 90% MeOH and 0.1% formic acid. 10 µl per sample was injected into a Waters Acquity UPLC System interfaced with an AB Sciex 6500 QTrap mass spectrometer (Sciex, ...
1) and interacts directly with caveolin-1, albeit through a totally different site independent of the caveolin-1 scaffolding domain (69, 70). Fourth, on a mass basis (nmol/mg protein), hepatocyte lipid rafts were found to be highly lipid-rich in total lipid and phospholipids as well as ...
Once at the OMM, proteins of the steroidogenic metabolon, which include TSPO and VDAC along with the IMM proteins ATPase family AAA domain- containing protein 3A and CYP11A1, facilitate the transport of cholesterol to the matrix side of the IMM, where cholesterol is converted to pregnenolone by...