Adenovirus-mediated small interfering RNA (siRNA) was employed to down-regulate the expression of c-Met gene in SCLC cell line (NCI-H446). MTT and colony formation assays were performed to detect in vitro proliferation of NCI-H446 cells. In vitro wound-healing and transwell invasion assays ...
结果:1.通过Western Blot方法检测,H446-Cav 1细胞株中Caveolin-1的表达明显高于NCI-H446和H446-neo细胞株.2.细胞凋亡检测实验的结果显示:在顺铂和UVR诱导后,细胞系H446-Cav1的细胞凋亡率较细胞系NCI-H446和细胞系H446-neo显著降低;而细胞系NCI-H446和细胞系H446-neo之间并无差异.3.Western blot检测表明:同细胞...
The IC(50) values in NCI-H460 cells were 94.37 g/mL (24h) and 20.89 g/mL (48h), whereas in NCI-H446 cells IC(50) values were 214.72 g/mL (24h) and 114.58 g/mL (48h). Annexin V-FITC/PI staining showed CYBYT could significantly induce apoptosis in NCI-H460 and NCI-H446 ...
方法:1.本实验室之前已经通过细胞转染的方法将Caveolin.1转染至人小细胞肺癌细胞系NCI-H446,并且建立了Caveolin.1稳定转染细胞株H446.Cavl,和空质粒稳定转染细胞株H446.neo,首先通过WesternBlot的方法检测三株细胞Caveolin・l的表达情况,验证转染率。2.通过顺铂(CDDP)和紫外照射(Ultravioletradiation,UVR)两种方法,即...
Only four of the 50 SCLC cell lines were TP53 wild type (NCI H2081, H446, H209, and H1522). RESULTS shRNA Knockdown of EZH2 Reduces Transcript and Protein Levels in SCLC The involvement of EZH2 in the RB1/E2F pathway prompted us to study the function of EZH2 in the context of cell...
Methods: NCI-H446 cell line was cultured in the presence of various concentrations (1(1000 ng/ml) of nicotine for 48 h. MTT was applied to evaluate the effect of nicotine on the growth of NCL-H446, small cell lung carcinoma (SCLC) cell line. After NCI-H446 cells were treated with 100...
The cell-lines tested are ranked from highest to lowest sensitivity: NCI-H82, NCI-H69, NCI-H1618, LX22 (ex-vivo cultured PDX), NCI-H1092, NCI-H446, SHP77 and NCI-H196; with EC50 values ranging from 18nM to 80nM. However, NCI-H526, NCI-H889, and SBC-5 were non-responsive ...
in human SCLC cell lines and in vivo models.Methods: Cytotoxicity assays: SCLC cell lines DMS 53, DMS 114, NCI-H69, NCI-H82, NCI-H196, NCI-H209, NCI-H211, NCI-H446, NCI-H526, NCI-H841, NCI-H889, NCI-H1048, and SHP-77 were tested with lurbinectedin dose range from 100 ...
Treatment of H446 spheroids with Teri (800; 1,600; and 3,200 nM) or Lur (800; 1,600; and 3,200 pM) had a very small effect on the level of total or phosphorylated RNA pol II. In contrast, the combination of Teri&Lur inhibited RNA pol II activity and increased the levels of ...