All cells were centrifuged and resuspended in 100 μL IMDM and seeded into 1 mL Methocult GF-M3434 methylcellulose and were cultured in a fully humidified atmosphere of 5% CO2 for 7 days prior to scoring. To confirm that erythroid colonies were hemoglobinized 2,7-diaminofluorene (Sigma-...