The phase diagram in Fig. 2b shows the proportion of the anisotropic phase as a function of the total CNC concentration in the suspension, which can be calculated as the ratio of the volume of the anisotropic phase to the total sample volume. In the biphasic regime, the suspension separates...
Here, we tested a pool of 188 Biolegend® TotalSeq™ C antibodies (TableS1) that were oligonucleotide-tagged to be compatible with 10 × Genomics 5′ sequencing. For titration, we used the antibodies at the recommended concentration (1 ×) and generated higher (2 ×) and lower...
Through nonlinear least-squares processing of the titration curve, the species present at the equilibrium are identified and the corresponding complexation equilibrium constants are determined. On the basis of the values of the protonation and complexation constants, a distribution diagram (% concentration...
For RNA virologists, the advent of recombinant DNA technology in the late 1970s made it possible to convert viral RNA genomes into cDNA clones, which could then be propagated as plasmids in bacteria for the genetic manipulation of RNA viruses.1The first RNA virus to be molecularly cloned was...
DeltaDiode laser and LED sources offer plug and play ease of use while delivering excellent performance and reliability for the most demanding TCSPC experiments. With available wavelengths from the UV to the NIR, these picosecond sources can operate at rates up to 100...
Phase diagram of cerium Cerium is especially interesting because of its variable electronic structure. The energy of the inner 4f level is nearly the same as that of the outer or valence electrons, and only small energy is required to change the relative occupancy of these electronic levels. Thi...
Flow diagram of community DNA extraction. Full size image Polymerase chain reaction (PCR) For the PCR analysis, the hypervariable region (V3-V4) of the 16S rRNA was targeted using bacteria-specific primers, namely 341F and 806R. The primers were tagged according to108. All the PCRs were ...
In recent decades, aqueous two phase systems have gained a lot of attention for extraction of different materials. In this work, an aqueous two phase system was made by polyethylene glycol 600 and potassium hydroxide and phase diagram were determined for this system. The experimental binodal data...
FIG. 2 illustrates, by way of example and not limitation, a block diagram of a pain management system.FIG. 3 illustrates, by way of example and not limitation, a block diagram of a pain management system comprising an implantable neuromodulator.FIGS. 4A-B illustrate, by way of example and...
FIG. 2. (a) A ribbon diagram of the CBD of IL-6R, showing the β-sheet arrangement of the two FnIII domains, and the cytokine binding loops L1 to L7. (b) the same as in (a) but rotated 90° with the loops facing up.