Schistosoma japonicum is a parasitic flatworm that causes human schistosomiasis, which is a significant cause of morbidity in China and the Philippines. Here we present a draft genomic sequence for the worm. The genome provides a global insight into the
A mainland strain of Schistosoma japonicum used for all experiments was originally maintained in Oncomelania hupensis snails and in New Zealand White rabbits. Six to 8-week-old BALB/c mice were purchased from the Songjiang Animal Facility of the Chinese Academy of Sciences of Shanghai. Seven weeks...
Fig.S4. Images of Schistosoma japonicum eggs under the fluorescence microscope. The eggs indicated no spontaneous fluorescence. Fig. S5. Blood glucose concentration vs. time curves of different treatments in the preliminary experiment. Data were presented as mean ± S.D. (n = 3). *p < ...
Using S. japonicum miracidia and S. turkestanicum miracidia as samples, the miracidia were identified under a fluorescence microscope by adding specific positive serum and the recombinant protein rSPG-EGFP. In addition, because the recombinant protein rSPG-EGFP contained the C3 region of SPG, ...
Then the cercariae on the cover slip were counted under a dissecting microscope and prepared for infection. Freshly shed S. japonicum cercariae on the cover slip were attenuated with ultraviolet rad...Bartley PB , Glanfield A , Li Y , Stanisic DI , Duke M , Jones MK et al . (...
japonicum. Thus, the functions of calpain need to be intensively identified and characterized in other human Schistosoma species to develop pan-inhib- itor and pan-vaccine against all species causing schisto- somiasis in both humans and animals. In this study, we identified and functionally ...
Aim To study the effects of PHA on the proliferation of cultured cells from adult Schistosoma japonicum. Methods By microscopy and electron microscopy, cultured cells treated in 333. 3g/ml PHA for 24 hours were observed. Results Some new lymphoboast--like cells were found among the degenerated ...
japonicum, which could be potentially valuable when gene functional analysis must be performed at larger worm stages. Previous studies showed that green fluorescence protein (GFP) could be expressed under the regulation of a CMV promoter in S. japonicum [11]. In this study, Cas9-ZsGreen-...
Liver granulomatous inflammation and fibrosis were the primary pathological changes observed during Schistosoma japonicum (S. japonicum) infection. In the present study, the characteristics of IL-9 were investigated in the liver of S. japonicum infection C57BL/6 mice. Immunofluorescence, qRT-PCR, and...
Schistosoma japonicum(日本血吸虫)is prevalent in Far East. In china, it is prevalent in Yangtze valley and south of Yangtze except Guizhou Province. The adults live in the portal vein system, causing liver cirrhosis(肝硬化)and portal vein hypertension syndrome(门脉高压征候群). Male and female ...