- 针对FBXO31敲除HEK293T细胞中表达GFP及其指定C端序列(C-term.)的蛋白稳定性测定。在测量前两天,细胞被转导表达野生型FBXO31或FBXO31(D334N)的载体,并使用流式细胞术进行测量。Y轴表示GFP荧光与共表达对照蛋白mCherry的比值。aa表示氨基酸。- 如d所述,针对ZMAT2 C端序列及其指定突变的蛋白稳定性测定。- ...
核纤层的持续存在阻碍染色质释放和染色体运动:为了研究核纤层持续存在对减数分裂的影响,研究人员将 GFP:CENH3 和 ASY3:RFP 报告构建体导入 rmf1 rmf2 和 ask1 突变体中。在减数分裂间期,突变体中的 CENH3 焦点定位与野生型相似。但在中期细线期,rmf1 rmf2 突变体中的染色质和着丝粒未能从核膜上释放并聚集,许多...
🔹图2显示了AXR6pro:AXR6-GFP在胚胎组织发育不同阶段的定位,包括皮层(G)、球形胚(H)、心形胚(I)、鱼雷(J)和弯曲子叶(K)阶段的胚胎表达。AXR6,也称为CULLIN1(CUL1),编码一种cullin蛋白,是SCF泛素连接酶复合物的一部分,参与介导对生长素和茉莉酸的反应。同型的抗生长素突变体在发芽后很快停止生长,缺乏根...
amino acids.e, Protein stability assay as indfor the ZMAT2 C terminus with the indicated mutations.f, Competitive proliferation assay measuring the impact of FBXO31 variant expression inFBXO31-KO HEK293T cells on cell fitness. The fraction of cells stably expressing GFP-linked cDNAs...
目的 克隆构建绿色荧光蛋白( GFP) / Akt 表达载体,观察其在鼠骨髓间充质干细胞(MSCs) 中的表达和定位,并探讨干细胞因子( SCF) 通过PI3-Akt 途径对转染p EGFP-C1/ Akt 的MSCs 中c-kit 、Akt 和VEGFmRNA及蛋白表达的影响。方法 采用酶切法将Akt 重组于GFP 表达载体中,经酶切序列鉴定后,将该重组质粒GFP/...
b Anti-GFP, -DMC1, and -UBQ11 antibodies were used to examine the ubiquitination level of DMC1-GFP immunoprecipitated by anti-GFP magnetic beads from tobacco leaves infiltrated with DMC1-GFP. GFP was included as a control; c Anti-FLAG, -DMC1, and -UBQ11 antibodies were used to examine...
pCMV5 BRG1-Flag was a gift from Joan Massague (Addgene plasmid # 19143), GFP-SMARCA4 was a gift from Kyle Miller (Addgene plasmid # 65391). W.W. and H.I. are American Cancer Society Research Scholar. This work was supported in part by the NIH grants to W.W. (R01GM089763 and...
干细胞生长因子或干细胞因子主要用于产生iPSC或由Jamaka或Thomson因子诱导的多能干细胞,例如使用5个慢III型CMV病毒,表达Yamanaka iPSC因子集(Oct4,Sox2,Nanog和Lin28)+GFP阳性对照。转分化将忽略干细胞运动场,但干细胞因子在转分化策略中起重要作用。 基因靶干因子SCF...
scfcp = kmalloc(sizeof(*scfcp), GFP_ATOMIC); if (!scfcp) { WARN_ON_ONCE(!IS_ENABLED(CONFIG_KASAN)); atomic_inc(&n_alloc_errs); allocfail = true; } else { scfcp->scfc_cpu = -1; scfcp->scfc_wait = scfsp->scfs_wait; scfcp->scfc_out = false; scfcp->scfc_rpc = false...
HA-Fbw7 expression lentiviral plasmids were constructed by subcloning the HA-Fbw7 cDNA fragment into pLenti-GFP-Puro vector (Addgene). The GST-F-box protein expression plasmids were obtained from Dr. J. Wade Harper. The 3XκB, mutant-3XκB, IL-6 and mutant-IL-6 NFκB-responsive luciferase...