7 Zemla, A., Thomas, Y., Kedziora, S., Knebel, A., Wood, N.T., Rabut, G., and Kurz, T. (2013). CSN- and CAND1-dependent remodelling of the budding yeast SCF complex. Nat. Commun. 4, 1641. https://doi.org/10. 1038/ncomms2628.8 Wu, S., Zhu, W., Nhan, T., Toth...
7 Zemla, A., Thomas, Y., Kedziora, S., Knebel, A., Wood, N.T., Rabut, G., and Kurz, T. (2013). CSN- and CAND1-dependent remodelling of the budding yeast SCF complex.Nat. Commun.4, 1641. https://doi.org/10. 1038/nc...
To determine whether COI1 also functions in an SCF complex, we have characterized Arabidopsis proteins that bind to COI1. An Arabidopsis cDNA expression library was screened in yeast for clones that produce proteins which can bind to COI1. We recovered two SKP1 homologues and a histone ...
Expression of this cDNA in yeast is able to rescue the cdc53-1 mutation (not shown) indicating that the protein can perform at least some of the functions of the yeast Cul1. We used the same strategy to identify a Skp1 cDNA that also rescues skp1 mutations in yeast (not shown). ...
between a given F-box protein and Skp1 [8]. Structure-function studies in yeast and mammals have demonstrated that the cullin functions as a scaffold in assembling the different subunits of the SCF complex. The cullin interacts at its carboxyl terminus with the RING domain protein Rbx1 to ...
However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood. In this study, we conducted yeast two-hybrid assays to further explore the interactions between P7-2 and 25 type I Oryza sativa SKP1-like (OSK) proteins, and found that P7-2 interacted with ...
Figure 3: SAP physically associates with components of the SCF complex. (a) The F-box motif of SAP is required for the interactions with ASK1 and ASK2 in yeast cells. The SAP protein contains a serine/glycine-rich domain, an F-box motif and a WD40-like domain. The indicated construct...
et al. Yeast Augmented Network Analysis (YANA): a new systems approach to identify therapeutic targets for human genetic diseases. F1000Res 3, 121 (2014). PubMed PubMed Central Google Scholar Watson, I. R., Irwin, M. S. & Ohh, M. NEDD8 pathways in cancer, Sine Quibus Non. Cancer...
There are ~700 FBPs in Arabidopsis, most of which are uncharacterized. TIR1 is among the best-studied plant FBPs and functions as a receptor for the plant hormone auxin. Here we use a yeast two-hybrid system to identify novel TIR1 mutants with altered properties. The analysis of these ...
For each IP, 1 pmol of peptide library was incubated with an equimolar amount of recombinant Strep-II-tagged FBXO31–SKP1 complex in 1 ml of binding buffer (200 mM NaCl, 25 mM HEPES pH 7.5, 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl ...