A stable HD-150Q cell line, containing a ecdysone-inducible pIND-tNhtt-150Q-GFP construct, has already been described.8 Cell-culture media and fetal bovine serum (FBS) were obtained from Gibco. Cells were maintained on Cell Death and Disease Implicating SCF in polyQ disease pathogenesis S ...
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基于WebAssembly 容器镜像的高性能腾讯云函数开发模版。A template project for building high-performance, portable, and safe serverless functions in Tencent Serverless Cloud Functions. - tencent-scf-wasm-runtime/yarn.lock at main · second-state/tencent-scf
Samples were mixed on ice for 30 min in 25 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5. Samples were plunged with various dilutions for optimal particle density. CAND1-CUL1-RBX1 was generated by incubating 10 μM CAND1 and 10 μM CUL1-RBX1 in the same condition above. 3.5 μL of ...
Calibration was done with Chymotrypsinogen (25 kDa), Ovalbumin (43 kDa), Albumin (67 kDa) and IgG (150 kDa). Full size image Figure 8 Antigen binding ELISA of soluble antibody fragments produced in E. coli using pOPE101 with 1 μg/well lysozyme coated per well. A 5 μM gelfiltration ...
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For co-immunoprecipitation analysis, cells were re-suspended in 1 ml lysis buffer (25 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 5% glycerol, protease inhibitor cocktail (Sigma S8830)), and after 1 h incubation at 4° C., cell lysate was clarified by centrifugation at 15000...
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