We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. Using this platform, we investigated whether fibrotic ECM scarring affected human skeletal muscle progenit...
Embryos or larvae that developed severe heart edema throughout the course of the experiment were excluded from analysis. For targeted cell ablation, the pro-drug Metronidazole (MTZ; Sigma-Aldrich #M3761) was dissolved in DMSO at 800 mM stock concentration and used at a final concentration of 2...
After 24 h, serum-free media was added to the insert to allow for chemotaxis, and cells in both the insert and bottom were treated with either DMSO control or G:T=1:1 for 48 h. Cells on the upper side of the insert were then removed using a cotton swab, and cells on the ...