研究者发现4名SARS-CoV-2感染者的血清或血浆对SARS-CoV-2 可溶性脯氨酸稳定的S蛋白胞外域(S2Pecto ), RBD和NTD 蛋白都有高反应性,其中D1989对SARS-CoV-2 NTD蛋白的反应性最高。随后研究者利用xCELLigence RTCA MP对D1989患者血清进行中和检测,结果发现即使在感染后90 天(患者已痊愈)的血清对VSV-SARS-CoV-2...
28、还提供了一种编码sars-cov-2刺突(s)糖蛋白或其衍生物或它们的片段的假型lv载体颗粒,其中该假型lv载体颗粒通过包括用选自以下的载体共转染宿主细胞的方法制备:pflap-iecmv-s2pδf-wprem(cncm i-5537),pflap-iecmv-s2p3f-wprem(cncm i-5538),pflap-iecmv-s2p-wprem(cncm i-5539),pflap-iecmv-...
三轮筛选产生了21个独特的结合SpikeS2P的纳米抗体,而且ACE2胞外结构域(ACE2-Fc)的二聚体构造体可降低这种结合。这些纳米抗体分为两类。第I类纳米抗体结合RBD并直接与ACE2-Fc竞争(图1B)。这一类的典型例子是纳米抗体Nb6,它可与SpikeS2P和RBD...
30、在另一个具体的实施方案中,编码sars-cov-2的祖先毒株的s蛋白的多核苷酸的天然序列包含seq id no:1的氨基酸序列的第986位赖氨酸残基突变为脯氨酸残基(k986p)和seq id no:1的氨基酸序列的第987位缬氨酸残基突变为脯氨酸残基(v987p),即突变2p在seq id no:19(s2p)中定义。包含所述突变2p的sars-cov-2的...
本发明以NDV LaSota病毒株为骨架载体,S2P蛋白基因插入到NDV病毒载体P基因和M基因之间,deltaRBDFc融合蛋白基因插入M基因和F基因之间,利用病毒反向遗传学技术拯救获得具有限制感染性NDV毒株用于疫苗株生产.相对于目前单个新冠S抗原或RBD抗原疫苗,本发明引入原始株S蛋白突变体S2P的编码序列和含Delta变异株S蛋白受体结合域RBD...
Samples for cryo-EM grids preparation were produced by mixing purified SARS-CoV-2 S2P spike (final trimer concentration of 0.33 mg/mL) with NTD-directed Fabs in a 1:9 molar ratio, followed by incubation on ice for 1 h. The final buffer for 1-87, 4-18 and 5-24 complexes was 10 mM...
SARS-CoV-2 S2P spike trimer proteins of the D614G and Omicron subvariants were generated by transfecting Expi293 cells with the S2P spike trimer-expressing constructs using 1 mg ml−1 polyethylenimine and then purifying from the supernatants 5 days post-transfection using Ni-NTA resin ...
conserved 25-residue peptide within the S2 stem helix127. CC40.8 around the stalk region of S2 produces a high atom density that may hinder protein refolding from the pre- to post-fusion state, and thus block viral entry. The binding motifs of B6, S2P6 and CV3-25 mAb overlap with the ...
SARS-CoV-2 S2P protein This paper N/A SARS-CoV-2 RBD protein This paper N/A SARS-CoV-2 Omicron RBD Sino Biological Cat#40592-V08H121 SARS-CoV-2 WT-S Acro Biosystem Cat#SPN-C52H9-500ug SARS-CoV-2 Omicron-S Sino Biological Cat#40589-V08H26 Bacterial and virus strains Authentic ...
SARS-CoV-2 Hexapro S, SARS-CoV S2P, and HKU1 S2P ectodomains were purified from clarified supernatants using a HisTrapHP column (Cytiva) and washed with 10 column volumes of 25 mM sodium phosphate pH 8.0 and 150 mM NaCl before elution on a gradient up to 500 mM imidazole. OC43 S,...