/点击蓝字[图片]关注我们 Immunity Advances ✦撰文丨WHY制版丨粥粥与其他冠状病毒类似,新冠病毒NP蛋白通过电荷作用将正链病毒基因组RNA压缩成螺旋核糖核蛋白(RNP)。在SARS-CoV-2感染后,NP蛋白与病毒基因组和M蛋白相互作用,提高亚基因组病毒RNA.....
(RNP) (2, 3). The SARS-CoV-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-...
总的来说,该研究解析了真实的SARS-CoV-2的分子组装,基于约2300个完整的病毒颗粒,在分子层面上提供了融合前和融合后的S蛋白结构、RNPs以及他们是如何装配病毒的。此外,还分析了天然S蛋白上的N-糖基化结构,重建后的病毒图谱由SARS-CoV-2真病毒的包含向上和向下...
病毒生命周期中的关键一步是将其基因组包装成新的病毒粒子。自结合和同源寡聚化是冠状病毒 SARS2-NP 的主要特性,它们对病毒 RNP 形成和核衣壳组装至关重要。该研究发现SUMOylation 对于SARS2-NP 自结合和相分离凝聚物的形成至关重要。此外,SUMO化的SARS2-NP导致与病毒RNA的结合和液-液相分离(LLPS)更强。因此...
为了揭示SARS-CoV-2RNA相互作用组,作者开发了一种健壮的核糖核蛋白(RNP)捕获协议,并鉴定了109个直接与SARS-CoV-2RNA结合的宿主因子。将RNP捕获到另一种冠状病毒HCoV-OC43上,揭示了冠状病毒RNA和宿主蛋白之间进化上保守的相互作用。转录组分析和敲除实验描述了17个抗病毒限制性商业惯例,包括ZC3HAV1,TRIM25,PARP12...
(RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence ...
全长MERS-CoV N蛋白与先导化合物P3结合后,会形成大的蛋白聚集体,并通过聚集体中的N-CTD(核衣壳(N)蛋白中的C末端域)自然形成拓扑封闭的八聚体,从而诱导N蛋白聚集而抑制MERS-CoV RNP(中东呼吸综合征冠状病毒核糖核蛋白复合体)的形成。 图5:与化合物P3结合后的蛋白聚集体。图片来源于参考文献1...
Specifically, we observed gradual increases in pseudoribosomal ribonucleoprotein (RNP) interactions that partially overlapped the trails of ribosomes, being likely involved in impeding translation elongation. Contemporaneously developed ribosome heterogeneity with predominantly dysregulated 5 S rRNP association ...
(β4-β2-β3-β1-β5-β6) form the palm and a protruding positively charged β-hairpin located between β2 and β3 forms the basic finger88(Fig.3b). A typical characteristic of the N protein is that it binds to viral RNA and forms a ribonucleoprotein complex (RNP).89In the N-...
核衣壳蛋白像线轴一样收纳RNA,将其有序缠绕,并组装成称为核糖核蛋白复合物(RNP)的高级结构,才能将RNA完整塞进病毒体内。在病毒发生膜融合及去组装后,它又能有条不紊地释放RNA,让它顺利进行复制。无DNA转录的纠错能力,容易发生突变...