基于此，团队对Jun12682进行了体内药效研究，结果显示，对SARS-CoV-2感染BALB/c小鼠，Jun12682口服给药不仅显著提高小鼠存活率，而且能够显著降低小鼠肺部病毒滴度和组织损伤，且降低多种炎症因子的表达 (图2)。图2 PLpro 抑制剂Jun12682的共晶结构和小鼠抗病毒活性 总括而言，基于新发现的Val70Ub的口袋，团队设计了...
Here we usedDrosophilato study the effect of individual SARS-CoV-2 proteins on the heart. Findings revealed that expression ofNsp6specifically in the fly heart causes pronounced structural and functional damage, in resemblance of the myocardial injury and functional abnormalities observed in patients wi...
SARS-CoV-2 infection and the overexpression of Nsp6, Nsp8, or M coherently upregulated genes associated with apoptosis and immune/inflammation pathways, whereas downregulated genes linked to heart contraction and functions. Global interactome analysis revealed interactions between Nsp6, Nsp8, and M ...
CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP16 is a small monomeric 298 amino acid protein with a characteristic fold of the class I methyltransferase (MTase) family with a seven-stranded B-sheet ...
在康复期COVID-19患者中，几乎能够检测到所有SARS-CoV-2蛋白特异性的CD4+ T细胞，其反应质量和水平与每种SARS-CoV-2蛋白的表达水平相关。除了S蛋白外，N、M、ORF3a和nsp3蛋白也能诱导相当水平的特异性CD4+ T细胞反应。在SARS-CoV-2的急性感染期和康复期，CD4+ T细胞的识别模式似乎是相似的，然而由于大多数...
SARS-CoV-2 infection of children leads to a mild illness and the immunological differences with adults are unclear. Here, we report SARS-CoV-2 specific T cell responses in infected adults and children and find that the acute and memory CD4+ T cell respon
下表中的26个SARS-CoV-2蛋白分别为:Nsp1、Nsp2、Nsp4、Nsp5、Nsp6、Nsp7、Nsp8、Nsp9、Nsp10、Nsp11、Nsp12、Nsp13、Nsp14、Nsp15、Spike (S)、E、N、M、Orf3a、Orf3b、Orf6、Orf7a、Orf8、Orf9b、Orf9c和Orf10,另外还有一个突变体Nsp C145A。
总括而言,基于新发现的Val70Ub的口袋,团队设计了一类结构新颖、高效的PLpro抑制剂,其中,Jun12682不仅能够有效抑制多种突变病毒株,且具有优异的体内活性,具有开发成为新型抗SARS-CoV-2新药的潜力。 美国罗格斯新泽西州立大学(Rutgers)副教授王俊,教授Eddy ...
Non-structural protein 16 (NSP16) and NSP10 are two of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of th...
Sera were collected from donor and recipient ferrets on the indicated days. Antibody responses against A/H1N1 virus (a) were measured by hemagglutination inhibition (HI) assay, whereas responses against SARS-CoV-2 (b) and SARS-CoV (c) were assessed using a nucleoprotein (NP) ELISA. Dotted li...