我们对来6位接种疫苗的健康人群,5位接种疫苗的康复人群,5位未接种疫苗的康复人群和4位感染新冠的人群进行单细胞测序分析,发现感染组扩增最多基因是IGHV3-33,而在接种疫苗组是IGHV3-23。感染组比接种疫苗的健康组有更多的克隆扩增和体...
IGHV3-53/3-66andIGHJ6BCR HC clonotype is present not only in the majority of convalescent patients from ancestral strains but also in normal human populations not exposed to SARS-CoV-2 and can neutralize the virus23. The frequent use of theIGHV3-53/3-66andIGHJ6gene pair in SARS-CoV-...
SARS-CoV-2, the virus responsible for COVID-19, has caused a global pandemic. Antibodies can be powerful biotherapeutics to fight viral infections. Here, we use the human apoferritin protomer as a modular subunit to drive oligomerization of antibody fragments and transform antibodies targeting SARS...
Furthermore, assembly of 716,806 SARS-CoV-2 sequences predicted emerging escape variants, which were also effectively neutralized. One of these broadly neutralizing potent antibodies, R40-1G8, is a IGHV3-53 RBD-class-1 antibody. Remarkably, cryo-EM analysis revealed that R40-1G8 has a ...
Since the start of the COVID-19 pandemic, SARS-CoV-2 has caused millions of deaths worldwide. Although a number of vaccines have been deployed, the continual evolution of the receptor-binding domain (RBD) of the virus has challenged their efficacy. In particular, the emerging variants B.1.1...
Since the COVID-19 pandemic onset, the antibody response to SARS-CoV-2 has been extensively characterized. Antibodies to the receptor binding domain (RBD) on the spike protein are frequently encoded by IGHV3-53/3-66 with a short complementarity-determining region (CDR) H3. Germline-encoded se...
SARS-CoV-2 Omicron subvariants have demonstrated extensive evasion from monoclonal antibodies (mAbs) developed for clinical use, which raises an urgent need to develop new broad-spectrum mAbs. Here, we report the isolation and analysis of two anti-RBD neutralizing antibodies BA7208 and BA7125 from...
This fusion process is mediated by the spike (S) glycoprotein, a 1,255-amino acid type I transmembrane protein2 that assembles into trimers on the virion surface to form the characteristic spike structure of the SARS-CoV. These spikes are essen- tial for the infection of the host cell ...
The FDA published, on 13 June 2022 [51], based on new pseudotyped virus-like particles and authentic virus data, that Bebtelovimab retains activity to Omicron subvariants BA.4 and BA.5. Therefore, Bebtelovimab seems to be the only EUA antibody broadly neutralizing SARS-CoV-2 and still ...
France 3 Institut Laue-Langevin, 71 Avenue des Martyrs, CS 20156, CEDEX 9, 38042 Grenoble, France Simple Summary: Nucleocapsid protein is one of the essential proteins for viral replication includ- ing the coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19) pneumonia leading...