Conclusion The development of a highly sensitive ELISA system for the detection of SARS-CoV-2 IgG antibodies is described herein. This system enabled a quantitative study of rSARS-CoV-2-N IgG antibody titers in COVID-19 patients, the occurrence of higher IgG antibody titers were found to be...
Humoral immunity in COVID-19 includes antibodies (Abs) targeting spike (S) and nucleocapsid (N) SARS-CoV-2 proteins. Antibody levels are known to correlate with disease severity, but titers are poorly reported in mild or asymptomatic cases. Here, we analyzed the titers of IgA and IgG against...
Fig. 1: Time course of vaccine-induced serum and mucosal anti-SARS-CoV-2 antibody titers in adult participants with or without previous SARS-CoV-2 infection. Anti-SARS-CoV-2 spike IgG titers in serum (a, b), anti-SARS-CoV-2 spike IgG titers in saliva (c, d), and anti-SARS-CoV-...
From June 2020, we recruited 10,039 participants to the project named the University of Tokyo COVID-19 Antibody Titer Survey (UT-CATS), and measured iFlash-SARS-CoV-2 IgM and IgG (YHLO IgM and IgG) titers in the collected serum. For the samples with increased IgM or IgG titers, we pe...
Final containers of IVIG/SCIG and CP donations were analyzed by commercial ELISA for anti-SARS-CoV-2 S1-receptor binding domain (RBD) IgG as well as microneutralization assay using a patient-derived SARS-CoV-2 (D614G) isolate. Neutralization capacities of 313 single plasma donations and 119 pla...
A, SARS-CoV-2 anti-S1/S2 IgG titers 2 to 4 weeks postvaccine in healthy controls (n = 40): mean (SD), 283 (100) AU/mL; patients with multiple sclerosis (MS) not treated (n = 23): mean (SD), 288.3 (113.8) AU/mL; patients with MS treated with ocrelizumab...
The SARS-CoV-2 coronavirus epidemic is hastening the discovery of the most efficient vaccines. The development of cost-effective vaccines seems to be the only solution to terminate this pandemic. However, the vaccines’ effectiveness has been questioned due to recurrent mutations in the SARS-CoV-2...
中和抗体(neutralizing antibodies,nAbs)滴度对于抗体保护细胞免受SARS-CoV-2感染至关重要。成人康复病例研究提示,针对SARS-CoV-2的体液免疫可能是短暂的,尤其是在轻症患者中。但是,近期的研究提示nAbs可维持长达6个月,正如季节性或SARS样冠状病毒感染...
Fig. 1: SARS-CoV-2 titer heterogeneity by symptoms. A IgG-RBD titer by reported symptom (values for individuals with multiple symptoms are shown for each symptom individually; LOS loss of smell, LOT loss of taste) (box extends from 25th to 75th percentile, whiskers show min and max, and...
and all samples with IgG titers ranging from 1:960 to ≥1:2880 neutralized SARS-CoV-2 infection. Overall, these results indicate that SARS-CoV-2 S-targeting IgG antibodies in SARS-CoV-2-infected patients neutralized authentic SARS-CoV-2 infection, and that the induced IgG antibody titer corre...