研究发现,SARS-CoV-2刺突蛋白的C端一个氨基酸残基可以控制其在细胞内的运输和纳入VLPs的过程。通过一系列实验和分析,揭示了这个氨基酸残基对刺突蛋白的稳定性、细胞膜定位和与细胞内膜蛋白的相互作用等方面起到关键作用。这项研究对于理解SARS-CoV-2病毒的感染机制和开发相关疫苗和药物具有重要意义。 为了确定SARS-CoV-...
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a recently identified coronavirus strain responsible for the Coronavirus Disease 2019 (COVID-19) and pandemic. SARS-CoV-2 is transmitted mainly through droplets and surface contact routes. The virus infects human cells through interactio...
图片:这张图显示了SARS-CoV-2表面的一系列刺突蛋白。每种颜色的抗体代表每个RBD社区可能的IgG-Spike结合模式。 出处: 经Hastie等人许可转载,来自《科学》(2021) 有些人认为疫情最糟糕的日子早就过去了,但新的病毒变异不断出现,疫苗标兵以色列破防,美国COVID-19病例再次升高,世界各地许多人将多年得不到COVID-19疫...
近日,上海交通大学陶生策团队联合华中科技大学范雄林团队等多家单位于Cellular & Molecular Immunology在线发表研究成果:Systematic evaluation of the IgG responses to SARS-CoV-2 spike protein-derived peptides for monitoring COVID-19 patients。该研究基于S蛋白全覆盖肽芯片,分析了大量的血清样本,鉴定出了几条具有高...
Widespread exposure to SARS-CoV-2 in wildlife communities The wildlife host range of SARS-CoV-2 is currently unknown. Here, the authors report evidence of infection in six common wild animal species (deer mouse, Virginia opossum, raccoon, groundhog, Eastern cottontail, Eastern red bat) out of ...
IgG antibodies induced by SARS-CoV-2 in patients with moderate-to-severe symptoms mainly target the S protein;1however, it is unclear which regions (RBD, NTD, or other fragments) of the S protein are targeted, or whether the induced antibodies show cross-reactivity and/or cross-neutralizing ...
利用这种方法,我们测量了XBB.1.5和BA.2 刺突中的突变如何影响ACE2结合(图2c,交互式图表可在 https://dms-vep.github.io/SARS-CoV-2_XBB.1.5_刺突_DMS/htmls/monomeric_ACE2_mut_effect.html https://dms-vep.github.io/SARS-CoV-2_Omicron_BA.2_刺突_ACE2_binding/htmls/monomeric_ACE2_mut_effect.ht...
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is the cause of coronavirus disease 2019 (COVID-19); a severe respiratory distress that has emerged from the city of Wuhan, Hubei province, China during December 2019. COVID-19 is
1. SARS-CoV-2 spike蛋白的基因组测序 2. ELISA 3. 慢病毒类假病毒构建 4. 假毒中和实验 5. 抗体介导的吞噬作用(ADCP) 6. 抗体介导的细胞毒作用(ADCC) 7. 抗体介导的补体沉积作用(ADCD) 8. 抗体介导的胞啃作用(ADCT) 9. Fcγ受体的ELISA实验 ...
使用HIV-1(人类免疫缺陷I 型病毒)为基础载体侵染293T细胞后,包装成SARS-CoV-2 (2019-nCoV) Spike假病毒,表面表达SARS-CoV-2 (2019-nCoV) Spike蛋白,并携带有荧光素酶报告基因,可用于侵染过表达ACE2的细胞,荧光素酶在细胞内表达。此假病毒无自主复制能力,具有安全性高、可操作性强等特点。