SARD1是植物免疫的激活子,会促进植物激素水杨酸SA的产量,激活防御基因的表达。SARD1基因本身是受到侵染的强烈诱导。本文研究了SARD1基因的转录调控。作者利用了酵母单杂试验来鉴定WRKY70。电泳迁移率变动分析法鉴定WRKY70的结合位点,并利用拟南芥的原生质体系统来研究其重要性。通过对wrky70突变体上病原菌的生长、SA浓度...
Two pathogen-induced transcription factors, SAR DEFICIENT1 (SARD1) and CAM-BINDING PROTEIN 60-LIKE G (CBP60g), regulate the expression of ICS1 and are required for pathogen induction of SA synthesis23,24,25. Following pathogen infection, SARD1 and CBP60g are recruited to the promoter of ICS...
主要通过ChIP-seq筛选到SARD1 and CBP60g的靶基因,通过ChIP-qPCR以及定量进行验证,发现其可以调控与SAR(systemic acquired resistance),ETI 及PTI正向调控相关基因;还发现SARD1 and CBP60g可结合一些免疫负调控基因,推测是与SA反馈抑制相关,避免自身免疫。另外还通过分析发现二者的结合元件GAAATTT,利用luciferase reporter...
Salicylic acid(SA) is an essential defence hormone in plants. Upon pathogen infection, induced biosynthesis of SA is mediated by Isochorismate synthase 1(ICS1), whose gene transcription is controlled mainly through two redundant transcription factors, SAR Deficient 1(SARD_1) and Calmodulinbinding ...
Loss of both CBP60g and SARD1 severely impacts the plants ability to produce SA in response to bacterial inoculation and renders the plant susceptible to infection. In an electrophoretic mobility shift assay CBP60g and SARD1 were shown to bind specifically to a 10mer oligonucleotide with the ...
1restru:m /n.(美)洗手间;公共厕所2//n.邮票;印章3./b'sard/prep.在……旁边在……附近4::(r)d/n.明信片5pa:(r)dn/v.原谅..请再说一遍6/ba:0ru:m/,/bae0ru:m/n.浴室洗手间7r/v.&n.仓促;急促8/sa'd3est/v.建议;提议★9/sta:f/,/staef /n.管理人员;职工10./grelp//n....
可以用如下方法:连结AO,由AD=DB得SADo=SDo,同理:S4cEo=SA4E,设SDo=x,S4cD=y,则Soo=x,So=y,由题意得S-Sec-30,Sac2a=30,可列方程组为:22x+y=301x+2y=30,解得x+y=20,可得四边形AD0E的面积为20.解答下面问题:如图3,D,F是AB的三等分点,E,G是C4的三等分点,CD与BE交于0,且S4=60,请计算...
SAR requires an information relay from primary infection to distal tissue and the initiation and maintenance of a self-maintaining phytohormone salicylic acid (SA)-defense loop. In spatial and temporal resolution, we show that calcium-dependent protein kinase CPK5 contributes to immunity and SAR. In...