主要通过ChIP-seq筛选到SARD1 and CBP60g的靶基因,通过ChIP-qPCR以及定量进行验证,发现其可以调控与SAR(systemic acquired resistance),ETI 及PTI正向调控相关基因;还发现SARD1 and CBP60g可结合一些免疫负调控基因,推测是与SA反馈抑制相关,避免自身免疫。另外还通过分析发现二者的结合元件GAAATTT,利用luciferase reporter...
(2012). Co-expression analysis identifies putative targets for CBP60g and SARD1 regulation. BMC Plant Biol. 12: 216.Truman,W. and Glazebrook,J. (2012) Co-expression analysis identifies putative targets for CBP60g and SARD1 regulation. BMC Plant Biol., 12, 216-232....
24). In the sard1 cbp60g double mutant, induction of ICS1 expression and SA synthesis is blocked24,25. SARD1 and CBP60g belong to the same protein family but are regulated differently, suggesting that they function in two parallel pathways to activate ICS1 expression23,24. CBP60g, but ...
Upon pathogen infection, induced biosynthesis of SA is mediated by Isochorismate synthase 1(ICS1), whose gene transcription is controlled mainly through two redundant transcription factors, SAR Deficient 1(SARD_1) and Calmodulinbinding protein 60-like g(CBP60g). Although these master transcription ...
CBP60gPlant immunitySARD1WRKY54WRKY70SARD1 and CBP60g are two master regulators in plant immunity. They are required for the constitutive defense responses in the Arabidopsis snc2-1D mutant, which carries a gain-of-function mutation in a receptor-like protein. Here we report that WRKY54 and ...
Negative regulation of resistance protein‐mediated immunity by master transcription factors SARD1 and CBP60gSalicylic acid (SA) is a plant defense hormone required for immunity. Arabidopsis NPR1 and NPR3/NPR4 were previously shown to bind SA and all three proteins were proposed as SA receptors. ...