1.摘要 在研究靶向毒素(一种针对独特细胞外靶点的药物、疗法或科学工具)的起源时,保罗·埃利希(Paul Ehrlich)的诺贝尔奖获奖作品和他的“魔法子弹”概念是一个合适的起点,使用靶向毒素进行分子神经外科手术已经成为一项重要的实践,它允许研究人员在消除神经元群体后观察生物体的变化。这种做法的一个主要例子是专门针对基...
SAP is aplant toxinthat inhibitsprotein synthesisby irreversibly inactivating ribosomes when expressed inmammalian cells. Hence, SAP has been used as a potent therapeutic gene for cancer gene therapy. Its expression in cancer cells after using a cancer-specific promoter or after selective delivery of...
靶向毒素(通常由化学连接或基因融合的抗体/靶向肽)为神经科学提供了强大的工具,它可以消灭神经系统中特定神经元群体的,然后被用于研究行为和功能。皂草素(Saporin)是一种理想的分子,因为它极耐高温和变性,结合后保持催化活性,并且缺乏结合链,无法自行进入细胞细胞质。因此,它有高活力、高效力、安全性和易于在实验室...
Saporin, which is extracted from Saponaria officinalis, is a protein toxin that inactivates ribosomes. Saporin itself is non-selective toxin but acquires high specificity after conjugation with different ligands such as signaling peptides or antibodies to some surface proteins expressed in a chosen...
The basic fibroblast growth factor-saporin mitotoxin acts through the basic fibroblast growth factor receptor. We have confirmed the hypothesis that a mitotoxin resulting from the conjugation of basic fibroblast growth factor and saporin exerts its cytotoxic effect ... DA Lappi,PA Maher,D Martinea...
In: Toxin-Induced Models of Neurological Disorders, Woodruff, M. and Nonneman, A., eds, Plenum Press, NY, 259–279. Google Scholar Walsh, T.J., Kelly, R.M. and Stackman, R.W., 1994, Strategies to limit brain injury and promote recovery of function. Neurotoxicology 15:467–476. ...
Biological and chemical characterization of basic FGF-saporin mitotoxin 1989, Biochemical and Biophysical Research Communications more Douglas A. Lappi, Darlene Martineau, Andrew Baird Biological and chemical characterization of basic FGF-saporin mitotoxin Biochemical and Biophysical Research Communications, ...
[100]Thakur, M.; Mergel, K.; Wenig, A.; von Mallinckrodt, B.; Gilabert-Oriol, R.; Dürkop, H.; Melzig, M.F.; Fuchs, H. Targeted tumor therapy by epidermal growth factor appended toxin and purified saponin: An evaluation of toxicity and therapeutic potential in syngeneic tumor bearing...
In order to produce sufficient quantities of fibroblast growth factor-saporin (rFGF-2-SAP) mitotoxin for preclinical evaluation in models of diseases such as cancer and restenosis, we have undertaken the large-scale expression, purification, and characterization of the recombinant molecule. The ...
Interestingly, the toxin was detected in nuclear extracts by immunoblotting, suggesting the possibility that saporin might induce direct DNA damage. However, confocal microscopy did not show a clear and consistent pattern of intranuclear localization. Finally, using the long-term culture-initiating ...