Deoxyribonuclease I (DNase I), Amplification Grade, for eliminating DNA during RNA purification procedures SuperScript™ III Reverse Transcriptase for high-temperature cDNA synthesis, ensuring high specificity and high yields of cDNA as well as more ...
Here's how PCR works:Denaturation: The DNA or RNA sample, which contains the target sequence to be amplified, is heated to a high temperature (usually around 94-98°C). This causes the double-stranded DNA to separate into two single strands, a process called denaturation.Annealing: The reac...
Seven screened samples were positive; four of the seven samples had volatile organic substances identified by second-column confirmat... RC Buchmiller - 《Ground Water Monitoring & Remediation》 被引量: 19发表: 1989年 The role of DNA double strand breaks in lonizing radiation-induced killing of...
(encoding adhesion protein, condensin and SMC5/6 complex protein) and Sycp genes (encoding the association complex), which play key roles in sister chromatid association or segregation, chromosome condensation, repair of DNA double-strand breaks, and homologous chromosome linkage, and their decreased...
second loop connecting the strands; annealing a first sequencing primer to the first loop oriented to sequence at least a portion of one strand of the fragment; and annealing a second sequencing primer to the second loop oriented to sequence at least a portion of the other strand of the ...
Standard/Strand-Specific RNA-Seq & Immuno-ProfilingSample Type: Total RNA (DNA-free) Sample Purity (OD260/OD280): 1.8-2.2 Recommended RIN:≥6.0 Recommended Quantity: >2 µg, >50 ng/µl Minimum Quantity: 250 ng Resuspension Buffer: Nuclease-free water Shipment Method: Dry ice...
This is possible because of the invention of a method in the 1980s called the polymerase chain reaction or "PCR", which allows an individual strand of DNA to be replicated many times. This creates thousands of copies until there is enough DNA to conduct tests. ...
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Strand displacement amplification 8. A method for amplifying a target nucleic acid sequence in a sample of biological material comprising the steps of: a) isolating nucleic acids from said sample; b) preparing double stranded fragments of the nucleic acids in the sam... GT Walker - US 被引量...
SNPs can be sequenced on either the forward strand (5′ to 3′) or reverse strand (3′ to 5′), which may result in the same SNP being reported as its complementary base pairs (A may be reported as T if sequenced from the reverse strand) [23]. While the effect allele can easily ...