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Global quantification of protein abundances in single cells could provide direct information on cellular phenotypes and complement transcriptomics measurements. However, single-cell proteomics is still immature and confronts many technical challenges. He
Afterward, the cells were sonicated for 20 min on ice in an UltraSonic Bath (Branson). The lysate was diluted 1:10 with HPLC-grade water for P1, and 1:5 with a dilution buffer (10% Acetonitrile (ACN) v/v, 25 mM Tris pH 8.5) for P2. For P1 and P2, the lysates were ...
In summary, we present GAWMerge, a protocol for integrating array and WGS genotype data to conduct GWAS with a case-only and public control design. This protocol overcomes previous obstacles to using public controls13. The ability to use WGS data for public controls (1) ensures complete overlap...
and 12.4 mL water were transferred to a 250 mL round-bottomed flask. The obtained solution was sonicated for an hour before growth was started. Under gentle stirring (200 rpm) and nitrogen flow, 3 mL of a 3 times diluted solution of TEOS in ethanol was added to the solution ...
(Supplementary Fig.12). As expected, due to matrix effect, the linearity was reduced to the range from 1 to 1000 ng/mL when negative pooled samples were used as diluent (Supplementary Fig.13). Some strategy to reduce matrix complexity such extra steps for analyte enrichment would be ...
For P1 and P2, cells were boiled at 95 °C for 5 min in a thermomixer at 800 rpm. Afterward, the cells were sonicated for 20 min on ice in an UltraSonic Bath (Branson). The lysate was diluted 1:10 with HPLC-grade water for P1, and 1:5 with a dilution buffer (10% Ac...
Thus, ratings, which bear product quality-related information [10,12] and are relatively hard for sellers to manipulate [9], rather than sales should be processed as high-scope cues by consumers. The framework further states that high-scope cues have direct and indirect effects on the ...