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Statistical testing was performed using unpaired two-sided t test. *P < 0.05; **P < 0.01. h Slot-blot assays of the overall chloroplast R-loop levels of 21-day-old plants. RNase H-treated DNA was used as the negative control. Experiments were repeated three times with similar ...
aMitogen-activated protein kinase (MAPK) cascades play important roles in mediating biotic and abiotic stress responses. In this study, we found that ZmMPK4 protein was predominantly localized in the nucleus. Semi-quantitative RT-PCR analysis revealed that the ZmMPK4 transcription in maize leaves ...
17A) and RNA were extracted for further real time RT-PCR test. The efficiency of bio-functional transfection of the polyplexes and lipoplexes in vitro was determined by quantifying the fluorescence intensity (FIG. 17B) and the miRNA expression level (FIG. 17C). In order to quantify the ...
Twenty-eight days after the gene introduction, total RNA was extracted from the cells. Using a probe and primers specific to osteocalcin gene, and a probe and primers specific to β-actin gene (Applied Biosystems), real-time RT-PCR was performed. The gene mRNA level relative to the β-ac...
Both the PCR and image analysis were optimized and shown to be in the linear range of detection. All amplicons have been sequenced to confirm the identity of the molecules and the primers used in previous studies [39], [43], [44]. All no-RT controls were negative for genomic DNA ...
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