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aeruginosa induced bacterial keratitis was examined daily up to 5 days post infection with a slit lamp microscope (Topcon, Tokyo, Japan) and scored as follows: a grade of 0–4 was individually assigned to criteria including area of opacity, density of opacity, and surface regularity. Briefly, ...
Strong light treatment was performed by exposing the colonies with a photon flux of 350 μmol m-2 sec-1. UV-B treatment was done using a Hi-Tech lamp (G25T8E, Japan) with UV-B 0.034 mW/cm2. All treatments were performed for 24 hours except for strong light that was performed for...
SOD (EC 1.15.1.1) activity measurement was based on the ability of the enzyme to inhibit the photochemical reduction in p-nitro-blue tetrazolium chloride (Sigma-Aldrich, St. Louis, MO, USA) in the presence of riboflavin in the light. One enzyme unit (U) of SOD represents the amount of ...