Five microliters of each supernatant was mixed with 200 μL of 0.25 mM bathocuproinedisulfonic acid disodium salt in 10 mM phosphate-buffered saline pH 7.4 and absorbance was read at 490 nm. Then, 50 μL of 0.5 mM CuSO4 was added to this reaction mixture, and incubated for 3 minutes at...
The internal correlations among these results, together with the influence of salt-processing, suggested that the effects of heating and newly generated surfactants during the salt-processing procedure were the primary causes of the changes in chemical composition and absorption characteristics, as well ...
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