Irradiated cells were definitely inhibited on Salmonella-Shigella agar or desoxycholate citrate agar, but only slightly, if at all, on brilliant green, bismuth sulfite or MacConkey agars. The highest count of irradiated cells of Salmonella newport by the MPN method was obtained with tetra-thionate...
7. Test for Salmonella Species— By means of an inoculating loop, streak portions from both the selenite-cystine and tetrathionate media on the surface of Brilliant Green Agar Medium, Xylose–Lysine–Desoxycholate Agar Medium, and Bismuth Sulfite Agar Medium contained in petri dishes. ...
Salmonellae can be isolated in the microbiology laboratory using numerous low-selective media (MacConkey agar, deoxycholate agar), intermediate-selective media (Salmonella-Shigella [SS] agar, Hektoen [HE] agar), and highly selective media (selenite agar with brilliant green). Salmonellae are oxidase...
Salmonellae were routinely cultivated in Luria-Bertani (LB) broth at 37°C or 42°C, on LB plates and on Brilliant Green plates supplemented with the appropriate antibiotics. Bacterial cultures to be used to infect chicks or mice were grown overnight to stationary phase in LB broth supplemented...
determined that SS Agar was inferior to Hektoen Enteric Agar and XLD Agar in the recovery of Shigella. Inhibitory action on coliforms and Gram-positive bacteria is obtained by the bile salts mixture and brilliant green in the medium. Sodium citrate inhibits Gram-positive bacteria. Neutral red is...
INTRODUCTION Salmonellae are Gram negative, short plump shaped rods, nonsporeforming, noncapsulated, aerobic and facultatively anaerobic organisms and classified under the family Enterobacteriaceae (OIE Manual, 2006). In 1919, Rettger reported a disease which was observed in ducklings from which he ...
After incubation, 0.1 mL of the enriched sample suspension was streaked onto brilliant green agar plates containing Km (25 µg/mL) as the ΔSE strains developed an inherent resistance to Km that allowed for the selection of the strains. After incubation for 24 h at 37°C, characteristic ...
is a live attenuated oral vaccine given to patients six years and older. The vaccine is administered in 4 doses on days 0, 2, 4, and 6. Vaccination should be completed one week before exposure. Boosters, with all 4 oral doses, should be completed every 5 years. Antimicrobials like pro...
typhimurium strains were grown at 37°C in tryptic soy broth (TSB, Difco) or on brilliant green agar (BGA, Difco) containing 25 μg/ml novobiocin (Sigma) and nalidixic acid (Sigma). S. typhimurium LT2 mutants were grown in Brain heart infusion medium (BHI, Difco) without antibiotics. ...
Brilliant Green Agar BPW: Buffered peptone water CFU: Colony-forming units CTB: Cholera toxin B subunit Dpc: Day post-challenge ELISA: Enzyme-linked immunosorbent assay FT: Fowl typhoid GM1: Monosialotetrahexosylganglioside HRP: Horseradish peroxidase IgG: Immunoglobulin G LB: Luria...