cerevisiae W303-1A F4. The protein was found to have a molecular weight of about 200kDa and belongs to Flo1-type. In addition, the recombinant yeasts including S. cerevisiae ZWA46 (Δ196-240), S. cerevisiae ZWA46 (Δ196-209) and S. cerevisiae ZWA46 (Δ210-240...
Saccharomyces cerevisiae yeast cells serve as a model to elucidate the bases of salt tolerance and potassium homeostasis regulation in eukaryotic cells. In this study, we show that two widely used laboratory strains, BY4741 and W303-1A, differ not only in cell size and volume but also in thei...
S. cerevisiaeW303-1 A (MATa his3Δ leu2Δ trp1Δ ura3Δ),Δdnm1(MATa his3Δ leu2Δ trp1Δ ura3Δ,dnm1::HIS3),Δfzo1(MATa his3Δ leu2Δ trp1Δ ura3Δ,fzo1::HPH), andΔhap2(MATa his3Δ leu2Δ trp1Δ ura3Δ,hap2::KMX6) strains were used in this study. TheΔdn...
产品名称Saccharomyces cerevisiaeMeyen ex E.C. Hansen 商品货号B215554 Deposited AsSaccharomyces cerevisiae Hansen, teleomorph ClassificationSaccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae ...
Chen C-L, Chen Y-C, Huang W-L, Lin S, Daugelavičius R, Rapoport A et al. A crucial role of mitochondrial dynamics in Dehydration Resistance in Saccharomyces cerevisiae. Int J Mol Sci [Internet]. 2021; 22(9). Tamura Y, Itoh K, Sesaki H, SnapShot. Mitochondrial Dynamics Cell. ...
The S. cerevisiae APP1 gene was cloned by PCR. A 3,024-bp DNA fragment that contains the entire coding sequence (1,764 bp) of APP1, the 5′-untranslated region (824 bp), and the 3′-untranslated region (436 bp) was amplified from W303-1A genomic DNA (forward, 5′-GATTATAAGCTT...
Purification of the nativeS. cerevisiaeNPC The yeast strainsS. cerevisiae(W303a and W303α) were used throughout the procedure. In theMlp1-PrA/Nup84-3FHstrain, endogenousMlp1andNup84were tagged with PrA preceded by TEV protease cleavage sequence (ENLYFQG) and 3× FLAG plus 10× His ...
. In order to isolate the gene encoding for glycerol specific carrier, an isogenic strain to W303- 1A, strain YSH642, carrying gpd1 gpd2 mutations unable to synthesize glycerol, was studied for further screening of a S. cerevisiae genomic library. ...
The W303 genetic background affects theisw2Δ mutant phenotype inSaccharomyces cerevisiae We performed detailed phenotypic analysis of the isw2Delta strains of the W303 genetic background and compared its results with those obtained previously i... P. Trachtulcová,I. Frydlová,I. Janatová,.....
cerevisiae poly(A) polymerases, the exosome together with its cofactors, and deadenylase complexes to shaping poly(A) tail length and abundance of all RNA biotypes. We used the nanopore direct RNA sequencing (DRS) technology25 to study the poly(A)+ RNA fraction, which improves the estimation...