S1 Nuclease Protection Assay End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10 µl water + olig S1 Nuclease Protection Assay End-label oligo (20-25 mer) 4 µl 5X T4 Kinase Buffer 5 µl [gamma-32P] ATP (7000 Ci/mmol) 10...
【答案】：是单链DNA探针与待测RNA形成DNA/RNA杂交双链，加入核酸酶S1专一性地降解未形成杂交体的DNA或RNA单链，DNA/RNA杂交双链则受到保护而不被降解的技术。
S1 nuclease protection assay (S1‐NPA)doi:10.1002/9783527678679.dg12271Wiley‐VCH Verlag GmbH & Co. KGaAThe Dictionary of Genomics, Transcriptomics and Proteomics
常用名核酸酶S1 来源于米曲霉英文名Nuclease S1 CAS号37288-25-8分子量N/A 密度N/A沸点N/A 分子式N/A熔点N/A MSDS美版闪点N/A 核酸酶S1 来源于米曲霉用途 核酸酶S1是一种降解单链DNA(ssDNA)和RNA的特异性内切酶。核酸酶S1还可以从双链DNA中去除突出的单链末端[1][2]。
DNA has been isolated from -irradiated yeast cells and then treated with the enzyme S1 nuclease. This enzyme cleaves DNA specifically at sites where localized denaturation has occurred and can therefore be used as a lesion probe to identify regions in the DNA where base-pairing has been disrupted...
中文名称:植物S1核酸酶ELISA试剂盒 英文名称:Nuclease,single-strandednucleate endo- CAS:37288-25-8 品牌: 一研 产地: 国产 保存条件: 2-8℃防潮避光 产品类别: Elisa Kit plant ELISA Kit 型号: EY-01E9884 【产品简介】: 产品名称 规格 型号 植物S1核酸酶ELISA试剂盒 96T/48T EY-01E9884 中文名称:植物...
Detection of single nucleotide polymorphisms by a gold nanowire-on-film SERS sensor coupled with S1 nuclease treatment[J]. Chemistry, 2011, 17(31): 8657-8662. [18]Draz MS, Lu X. Development of a Loop mediated isothermal amplification(LAMP)- surface enhanced raman spectroscopy (SERS)assay for...
英文名称Nuclease S1 from Aspergillus oryzae 单位定义One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6. 生化机理从黑曲霉(Aspergillus oryzae)中分离出的核酸酶 S1 对单链 DNA 和 RNA 的磷酸二酯键具有内切和外切水解活性,可产生 5'-phosphomononucleotide 和 5'...
样本:Nuclease,single-strandednucleate endo- 检测方法:ELISA 应用:酶联免疫试剂盒 检测限:0.3125ng/mL10ng/mL 货号:GOY-E965459 是否进口:是 尘埃芽孢杆菌 营养琼脂(NAP)[肉汤琼脂平板][空气平皿]70mm 头状秃马勃 藤黄色链霉菌 巧克力微杆菌 克雷伯菌冻干粉 ...
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