-整形美容领域:ADSCs在整形美容领域也有着重要的应用。通过移植ADSCs可以改善皮肤质地和光滑度,填补面部皱纹和凹陷,提升面部轮廓和紧致度。此外,ADSCs还可用于乳房重建和发际线调整等美容手术中。-免疫康愈领域:ADSCs具有免疫调节作用,可用于康愈某些自身免疫性疾病和炎症性疾病。例如,ADSCs可以通过减少炎症反应...
1. 功能强化:无血清培养基可能通过模拟体内微环境,上调ADSCs的特定基因表达,增强其存活能力和旁分泌活性。2. 外泌体富集:研究表明,无血清培养的ADSCs释放的外泌体数量更多且活性更强,其中携带的抗凋亡物质对肾小管保护更显著。3.协同修复:除抗凋亡作用外,ADSCs还可通过促进血管生成、抑制纤维化和调节免疫...
摘要:目的 探讨大鼠ADSCs、BMSCs、PMSCs诱导成骨后在体外环境下的抗原性差异。方法 体外培养8周龄SPF级近交系Wistar大鼠ADSCs、BMSCs、PMSCs,对细胞进行分离培养,采用CD分子表面标志物鉴定细胞并定向诱导为成骨样细胞,碱性磷酸酶染色、钙结节染色检测细胞成骨分化能力;与同种异体SPF级近交系WKY大鼠脾淋...
为缓解组织工程骨形成及血管生成缓慢的问题,本研究结合课题组前期的成果,探究负载P24活性肽的巯基化壳聚糖/纳米羟基磷灰石支架与ADSCs及HUVECs共培养在体内,外对骨质疏松性骨缺损的修复情况.研究方法: (1)根据前期研究的方法,将P24活性肽与CS/nHA支架复合,制备出负载P24活性肽的巯基化壳聚糖/纳米羟基磷灰石支架(CS-...
目的 为制备血管化胰岛,分离、培养脂肪来源干细胞(adipose derived stem cells,ADSCs),观察细胞共培养条件下,ADSCs对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVECs)增殖及成血管化功能的促进作用并探讨其机制。方法 采用胶原酶消化...
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Here, we evaluated the potential of EVs derived from healthy volunteer's ADSCs for the management of AD, finding that EVs entered the brain quickly and efficiently following intranasal administration, exerted powerful neuroprotective and neurogenesis effect, and rescued memory loss in APP/PS1 ...
(ADSCs). However, miR-26a functions oppositely in osteogenic differentiation of BMSCs and ADSCs, suggesting distinct post-transcriptional regulation of tissue-specific MSC differentiation. However, the molecular basis is largely unknown. Here, we report that the function of miR-26a is largely ...
19PCR仪Eppendorf美国二、实验方法原代ADSCs分离传代SD大鼠10只断颈法处死分别以0活力碘及75酒精浸泡消毒皮肤取下腹部正中切口至深筋膜向两侧的腹股沟区分离暴露双侧腹股沟脂肪垫尽量去除筋膜组织、淋巴结、小血管。含双抗的DHank’s液冲洗3次青霉素100Uml、链霉素100gml移入培养皿中剪碎成为1mm3组织Hank’s再清洗...
For preparation of vascularized islets, to isolate and culture human adipose derived stem cells, investigate the role of adipose derived stem cells (ADSCs) in promoting the proliferation and vascularization of human umbilical vein endothelial cells (HUVECs) co-cultured in vitro, and explore its mech...