Palmitoyl protein thioesterase (PPT1), a liposomal resident, was the first enzyme shown to catalyse the removal of fatty acids from proteins. Two cytoplasmic APT enzymes were subsequently identified that also act as lysophospholipases and are thus named LYPLA1/APT1 and LYPLA2/APT2.24Some eviden...
100% of −NH2OH acyl-RAC bound 1% of input (In) protein, protein were analyzed by 1W0e0s%teornf +blNotHti2nOgHwiatchyal-nRaAnCti-bSoOuDnd1 protein, and antibody. S-acylated in the human spinal cord tissues. CCS S-acylation was also evident by acyl-RAC in HEK293 cells (...
Protein S-acylation is a reversible post-translational modification that modulates the localization and function of many cellular proteins. S-acylation is mediated by a family of zinc finger DHHC (Asp-His-His-Cys) domain–containing (zDHHC) proteins encoded by 23 distinct ZDHHC genes in the human...
Here we propose the term "switching antibody" for an antibody that triggers deacylation of an intermediate enzyme-inhibitor complex by switching the enzyme-inhibitor reaction from the major pathway of stable acyl complex formation to an alternative pathway of cleavage of the inhibitor as a substrate...
(III) and (IV) are immunological adjuvants which intensify antibody prodn. and influence delayed hypeisensitivity reactions. They are non-arthrogenic, and exists their adjuvant effect (which is greater than that of the parent peptides in aq. soln. or suspension without the need to administer ...
(III) and (IV) are immunological adjuvants which intensify antibody prodn. and influence delayed hypeisensitivity reactions. They are non-arthrogenic, and exists their adjuvant effect (which is greater than that of the parent peptides in aq. soln. or suspension without the need to administer ...
Moreover, by harnessing the caged acylation reactivity of DBCO, site﹕pecific conjugation to an IgG antibody was achieved. Abstract Strain﹑romoted azide–alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper‐free bioorthogonal reactions. Reported here is the efficient ...
In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaging facilitated by silver catalysts, rendering site-specific conjugation of an IgG antibody by a Fc-targeting peptide.doi:10.1002/anie.202009408W.Shi...
In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaged by silver catalysts that renders site﹕pecific conjugation of IgG antibody via an Fc‐targeting peptide.doi:10.1002/anie.202009408...
Next we used acyl-RAC to examine SOD1 and CCS S-acylation levels in transgenic mouse models of ALS. Mouse spinal cord lysates from two G93A SOD1 mice (5.5 months of age) were first examined by Western blot analysis with either the anti-SOD1 or anti-CCS antibody (Supplemental Fig. S2)...