Palmitoyl protein thioesterase (PPT1), a liposomal resident, was the first enzyme shown to catalyse the removal of fatty acids from proteins. Two cytoplasmic APT enzymes were subsequently identified that also act as lysophospholipases and are thus named LYPLA1/APT1 and LYPLA2/APT2.24Some eviden...
The extracted total proteins were separated by 10% (w/v) SDS-PAGE gel and detected by immunoblotting with anti-phospho-p44/p42 MAPK antibody (Cell Signaling, 9101, dilution, 1:1000). Bacterial growth assays Bacterial growth was performed using flood inoculation of seedlings46. Generally, 50...
The samples were detected via SDS-PAGE and immunoblotting with an anti-GFP antibody (TransGen Biotech, HT801-01). Virus infection For BSCTV inoculation, 1.8 copies of the BSCTV genome in the pCambia1300 vector were used (Lai et al. 2009). The BSCTV plasmid was transformed into ...
Biotinylated proteins were revealed by incubating the membrane with NeutrAvidin-IR800, whereas the GST-tagged proteins were detected with an anti-GST antibody (IR680). Positions of the molecular mass markers (in kilodalton) are indicated on the right side of the membranes. C, schematics of the ...
Site-Specific Identification of Protein SAcylation by IodoTMT0 Labeling and Immobilized Anti-TMT Antibody Resin EnrichmentDownload: Download high-res image (101KB) Download: Download full-size imageJian CaiMing SongMing LiMichael MerchantFrederick Benz...
Abs (Babco, Berkeley, CA; Covance MMS 118P) and anti-His6 monoclonal Abs (H1029; Sigma, St. Louis, MO) were used at dilutions of 1:5,000 and 1:3,000, respectively, together with blotting grade HRP-conjugated goat anti-mouse IgG (Bio-Rad, Hercules, CA) as a secondary antibody. ...
Methods: We used the high-affinity antibody enrichment combined with high-resolution LC-MS/MS analysis to systematically investigate the global proteomic characteristics of the three acidic lysine acylations in Mycobacterium smegmatis. Subsequently, we employed in vitro enzymatic assays...
In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaging facilitated by silver catalysts, rendering site-specific conjugation of an IgG antibody by a Fc-targeting peptide.doi:10.1002/anie.202009408W.Shi...
Moreover, by harnessing the caged acylation reactivity of DBCO, site﹕pecific conjugation to an IgG antibody was achieved. Abstract Strain﹑romoted azide–alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper‐free bioorthogonal reactions. Reported here is the efficient ...
(III) and (IV) are immunological adjuvants which intensify antibody prodn. and influence delayed hypeisensitivity reactions. They are non-arthrogenic, and exists their adjuvant effect (which is greater than that of the parent peptides in aq. soln. or suspension without the need to administer ...