Data were normalized by the number of cells used in the assay (0.8 million in cell monolayer minus an estimated 20% cell loss during the washing steps). Intracellular amino acid levels were calculated by averaging the sum of unlabelled and heavy-labelled amino acid over all the timepoints. ...
CLIMP-63 has been shown to undergo S-acylation on its sole cytosolic cysteine residue, Cys-10033,37. It has only one other cysteine, Cys-126, which is located on the luminal membrane boundary of the transmembrane domain. To study CLIMP-63 S-acylation in-depth, we generated a HeLa cell ...
analogues: Ispinesib, S-Trityl-L-cysteine (STLC), and Arry-520, which are classified into the loop L5 binding allosteric suppressors, while GSK-1, PVZB1194, and BRD9876, particularly inhibits Eg5 by binding the helix-α4/6 region of the motor domain, categorized as Eg5 ATP-binding riv...
analogues: Ispinesib, S-Trityl-L-cysteine (STLC), and Arry-520, which are classified into the loop L5 binding allosteric suppressors, while GSK-1, PVZB1194, and BRD9876, particularly inhibits Eg5 by binding the helix-α4/6 region of the motor domain, categorized as Eg5 ATP-binding riv...
To perform electron microscopy imaging, Fabs were produced by digesting recombinant chromatography-purified IgGs using resin-immobilized cysteine protease enzyme (FabALACTICA, Genovis). The digestion occurred in 100 mM sodium phosphate and 150 mM NaCl pH 7.2 (PBS) for around 16 h at ambient tempera...
Anti N-acetyl-cysteine (carrier conjugated) N-乙酰半胱氨酸(载体偶联)抗体 品牌:Cosmo Bio 阿拉伯木聚糖[中黏度) Arabinoxylan (Med Vis) – 3g 货号:P-WAXYM Megazyme中国代理商 scientz08-II非接触式超声波细胞粉碎机-新芝超声波细胞粉碎机 IL-36alpha (aa 6-158) (human) (rec.) (untagged) 品...
The term “polypeptide” relates to a protein made up of any one of the natural or synthetic amino acids and their equivalents. Common amino acids, for example, are Alanine (Ala), Aspartic acid (Asp), Cysteine (Cys), Glutamic acid (Glu), Phenylalanine (Phe), Glycine (Gly), Histidine (...
Each peptide was individually conjugated with BSA using Sulfo-SMCC (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions. Briefly, BSA was activated by Sulfo-SMCC at a molar ratio of 1:30, followed by dialysis in PBS buffer. The peptide with cysteine was added at...
Our results revealed that LL-37 bound to SGG and its anionic lipid analog, sulfogalactosylceramide (SGC), as well as phosphatidylserine (also negatively charged), all immobilized separately in a well of a microtiter plate, in a specific manner. Kd values of the binding of LL-37 to these ...
(A) Both the potency and the efficacy of CCL5 are significantly increased in GAG-minus cells compared to GAG-plus cells in the absence of C21TP81-111boosting. Thus, although boosting persists in the GAG-deficient cells, it is limited how much boosting can occur when signaling in the ...