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followed by end repair and ligation of blunt adapters using SMRTbell Template Prep Kit 1.0 (Pacific Biosciences). The library was purified with AMPure PB beads and size selected
markers (Additional file8) suggesting that there may be recombination coldspots in the genome. To estimate the physical distance between recombination events across the genome, the cumulative scaffold length per recombination bin was calculated (Fig.2c). Whilst 91.3 % of bins were associated with a...
followed by end repair and ligation of blunt adapters using SMRTbell Template Prep Kit 1.0 (Pacific Biosciences). The library was purified with AMPure PB beads and size selected